|
| Status |
Public on Oct 01, 2013 |
| Title |
K14-Cre;Tgfbr2 fl/fl Mutant Posterior Palate 2 |
| Sample type |
RNA |
| |
|
| Source name |
E15.5 K14-Cre;Tgfbr2 fl/fl Conditional Knockout Mutant Mouse Model
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
genotype: K14-Cre;Tgfbr2 fl/fl
tissue: Posterior Palate
age: E15.5
gender: Not determined
|
| Treatment protocol |
Mice were maintained under standard IACUC protocols.
|
| Growth protocol |
Standard IACUC protocols were used
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Embryo was harvested at embryonic day 15.5 of development. Total RNA was extracted with the Qiagen RNeasy Plus Mini Kit according to manufacturer's protocol from anterior or posterior palatal tissue, from either mutant or control mice as designated.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix cRNA labeling protocol was used.
|
| |
|
| Hybridization protocol |
Standard Affymetrix hybridization protocol was used.
|
| Scan protocol |
Standard Affymetrix scanning protocol was used.
|
| Description |
Whole genome transcriptome profile of murine posterior palatal tissue, collected at E15.5, from mutant mice with a conditional inactivation of Tgfbr2 in epithelial cells.
|
| Data processing |
Samples were processed by RMA normalization and log2-scaled expression values were generated through the WebArray tool (URL: http://www.webarraydb.org/webarray/index.html) with default options. p-values for significance testing were adjusted using the SPLOSH (Spacings LOESS Histogram) method as implemented by WebArray.
|
| |
|
| Submission date |
Apr 19, 2013 |
| Last update date |
Oct 01, 2013 |
| Contact name |
Richard Pelikan |
| Organization name |
Oklahoma Medical Research Foundation
|
| Department |
Genes & Human Disease Research Program
|
| Lab |
Gaffney Lab
|
| Street address |
825 NE 13th St
|
| City |
Oklahoma City |
| State/province |
Oklahoma |
| ZIP/Postal code |
73104 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE46211 |
Gene expression profiling of anterior and posterior palatal tissue from Tgfbr2 mutant mouse models |
|
