|
| Status |
Public on Apr 23, 2014 |
| Title |
PAI-1 knockout mouse heart rep3 |
| Sample type |
RNA |
| |
|
| Source name |
12 m old PAI-1 knockout mouse heart
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype/variation: PAI-1 KO
age: 12 months
tissue: Heart
|
| Treatment protocol |
All heart and kidney tissue samples were collected from 12 months old wild type and PAI-1 knockout mice. The hearts and kidneys were snap frozen and stored at -80oC for RNA and biochemical analysis.
|
| Growth protocol |
PAI-1 knockout (PAI-1KO) and wild-type (WT) mice on a C57BL/6 background were purchased from Jackson laboratory (Bar Harbor, ME) and were maintained for 12 months. All mouse protocols were approved by the Animal Care and Use Committee of Northwestern University (Chicago, IL).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from hearts and kidneys derived from three PAI-1 knockout (12- month old) and three wild-type mice (12-month old) using RNeasy Fibrous Tissue Mini Kit (Qiagen, Valencia, CA) following the manufacturer’s instructions. The quality of RNA (RNA Integrity, RIN) in all 12 samples (3 wildtype hearts; 3 PAI-1 KO hearts; 3 wildtype kidneys; and 3 PAI-1 KO kidneys) was checked using the bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 3
KO_HT2
|
| Data processing |
The data were normalised using the default quantile normalisation with R/bioconductor package lumi
|
| |
|
| Submission date |
Apr 23, 2013 |
| Last update date |
Apr 23, 2014 |
| Contact name |
Chunfa Jie |
| E-mail(s) |
cjie.jhmi@gmail.com
|
| Phone |
3124043831
|
| Organization name |
Des Moines University
|
| Street address |
3200 Grand Ave
|
| City |
Des Moines |
| State/province |
IA |
| ZIP/Postal code |
50266 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE46320 |
Global Gene Expression Profiling in wildtype and PAI-1 Knockout Murine Heart and Kidney. |
|
