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Sample GSM1138455 Query DataSets for GSM1138455
Status Public on Jun 09, 2013
Title Neuron_3
Sample type RNA
 
Source name NSC derived Neuron
Organism Mus musculus
Characteristics strain: C57BL/6
cell type: differentiated neuronal progeny
tau-redstar expression: Yes
cell type: differentiated neuronal progeny
Treatment protocol Differentiation to neurons was performed in N2B27 for four days, followed by a replating step in Neurobasal medium supplemented with B27. Neurons were then FACS purified based on RedStar expression driven by the neuronal specific Tau promoter
Growth protocol NSCs were derived according to monolayer protocols (Ying et al 2003) and maintained in Euromed-N medium supplemented with N2 and FGF2/EGF.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the Qiagen RNA easy columns according to manufacturers protocols
Label Biotin
Label protocol RNA was labelled using a TotalPrep RNA Amplification Kit (Ambion).
 
Hybridization protocol Standard Illumina hybridization protocol to Illumina Mouse Ref-8 v2 BeadArrays.
Scan protocol Standard Illumina scanning protocol.
Description 4639127051_H
Data processing The data were normalized using quantile normalization using the Bioconductor BeadArray library in R. Differential expression was calculated using the limma library.
 
Submission date May 09, 2013
Last update date Jun 09, 2013
Contact name Matt Burney
Organization name King's College London
Street address 125 Coldharbour Lane
City London
ZIP/Postal code SE5 9NU
Country United Kingdom
 
Platform ID GPL6885
Series (2)
GSE46791 An epigenetic signature of developmental potential in neural stem cells and early neurons [expression]
GSE46793 An epigenetic signature of developmental potential in neural stem cells and early neurons

Data table header descriptions
ID_REF
VALUE log2 quantile-normalized signal intensity

Data table
ID_REF VALUE
ILMN_1250052 6.362323253
ILMN_3122480 5.977336878
ILMN_2599935 8.691621248
ILMN_2675543 6.27656003
ILMN_2686883 6.158876445
ILMN_2751818 6.413974571
ILMN_2728634 6.752701008
ILMN_3040515 6.28259468
ILMN_2711608 6.208231815
ILMN_1232875 6.367787703
ILMN_1258507 6.280514377
ILMN_2746142 6.188845413
ILMN_1253709 6.118196765
ILMN_1231129 11.22004638
ILMN_2711788 6.24531553
ILMN_2707099 6.184640848
ILMN_2942000 6.598053213
ILMN_2642403 11.76118645
ILMN_2935636 6.395037833
ILMN_1242024 6.220859903

Total number of rows: 25697

Table truncated, full table size 624 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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