|
| Status |
Public on Jun 21, 2013 |
| Title |
3rd instar neuroblast replicate 1 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Dam-Pol II processed DNA from 3rd instar larval brains expressing LT3-Dam-Pol II in neuroblasts
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype/variation: w-; insc-GAL4/+; tub-GAL80ts/UAS-LT3-Dam-Pol II
cell type: neuroblast cells
|
| Treatment protocol |
Brains were dissected from 3rd instar larvae
|
| Growth protocol |
Embryos were collected for 4-6 hours at 25°C, shifted to 18°C for 2 days, hatched larvae were transfered to a food plate for 5 days at 18°C, shifted to 29°C for 1 day.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Tissues were dissected and placed in 180ul of PBS with 100mM EDTA before samples were processed as previously described (Choksi et al., 2006)
|
| Label |
Cy5
|
| Label protocol |
Labeling has been done using the Nimbelgen Dual-Color Labeling Kit based on a Klenow enzymatic reaction following manufacturer's protocol
|
| |
|
| Channel 2 |
| Source name |
Dam-control processed DNA from 3rd instar larval brains expressing LT3-Dam in neuroblasts
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype/variation: w-; insc-GAL4/+; tub-GAL80ts/UAS-LT3-Dam
cell type: neuroblast cells
|
| Treatment protocol |
Brains were dissected from 3rd instar larvae
|
| Growth protocol |
Embryos were collected for 4-6 hours at 25°C, shifted to 18°C for 2 days, hatched larvae were transfered to a food plate for 5 days at 18°C, shifted to 29°C for 1 day.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Tissues were dissected and placed in 180ul of PBS with 100mM EDTA before samples were processed as previously described (Choksi et al., 2006)
|
| Label |
Cy3
|
| Label protocol |
Labeling has been done using the Nimbelgen Dual-Color Labeling Kit based on a Klenow enzymatic reaction following manufacturer's protocol
|
| |
|
| |
| Hybridization protocol |
Hybridisation has been done with HX1 mixer following Nimblegen's procedures
|
| Scan protocol |
Arrays were scanned on an Axon 4000B scanner following Nimblegen guidelines
|
| Description |
Dam-Pol II / Dam-control in 3rd instar neuroblasts
|
| Data processing |
The Dam-Pol II/Dam log2 ratios were median normalised using NimbleScan software (v.2.6, Nimblegen).
|
| |
|
| Submission date |
May 09, 2013 |
| Last update date |
Jun 21, 2013 |
| Contact name |
Tony David Southall |
| E-mail(s) |
t.southall@imperial.ac.uk
|
| Organization name |
Imperial College London
|
| Department |
Life Sciences
|
| Lab |
Southall lab
|
| Street address |
South Kensington Campus
|
| City |
London |
| ZIP/Postal code |
SW7 2AZ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL15641 |
| Series (1) |
| GSE46803 |
Profiling of RNA Pol II occupancy in Drosophila neural stem cell populations using TaDa (Targeted DamID) |
|
