|
| Status |
Public on Dec 08, 2014 |
| Title |
CON_sample 6 |
| Sample type |
RNA |
| |
|
| Source name |
Heart_CON
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Wistar
gender: Males
bw (body weight): 160-180g
condition: CON
tissue: Right ventricular wall
|
| Treatment protocol |
RNA was extracted out the right vetricular wall of the heart, Rats where sham operated or subjected to pulmonary atery banding,
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA
|
| Label |
Biotin
|
| Label protocol |
The Affymetrix GeneChip WT Sense Target Labeling and Control Reagents kit (P/N 900652) was used for the preparation of labelled cDNA from 500ng of total RNA without rRNA reduction
|
| |
|
| Hybridization protocol |
Hybridisation of 5.5ug labelled cDNA was done overnight for 17 hours, at 60 rpm, at 45ºC in a Hybridisation Oven 640 (Affymetrix). The protocol was conducted as described in the Affymetrix Whole Transcript (WT) Sense Target Labeling Assay Manual
|
| Scan protocol |
Washing and staining of the arrays was done on a Affymetrix 450 fluidics stations using the protocol FS450_0001, as described in the Affymetrix Whole Transcript (WT) Sense Target Labeling Assay Manual. Arrays where then scanned on an Affymetrix 3000 7G scanner, as described in the Genechip Expression Analysis Technical Manual
|
| Description |
Right ventricular wall
|
| Data processing |
The data where normalized using RMA normalisation strategies using the MADMAX analysis pipeline pmid:21778530
|
| |
|
| Submission date |
May 13, 2013 |
| Last update date |
Dec 09, 2014 |
| Contact name |
vincent bloks |
| Organization name |
UMCG
|
| Department |
Pediatrics
|
| Lab |
Pediatrics
|
| Street address |
Hanzeplein1
|
| City |
Groningen |
| ZIP/Postal code |
9713GZ |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL11534 |
| Series (1) |
| GSE46863 |
Clinical symptoms of right ventricular failure in experimental chronic pressure load are associated with progressive diastolic dysfunction |
|
