|
| Status |
Public on Oct 16, 2013 |
| Title |
SCI + delayed VHC graft 2dpi, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
5mm segment of Spinal cord
|
| Organism |
Rattus norvegicus |
| Characteristics |
treatment: VHC graft
time graft: delayed
time extraction: 2 days post graft
|
| Treatment protocol |
vehicle (VHC), mesenchymal stromal cells (MSC) or olfactory ensheathing cells (OEC) transplantation after spinal cord injury
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Spinal cord segment (5mm long) centered in the contusion epicenter was harvested and maintained in RNA-latter solution (Qiagen, Barcelona, Spain). The samples were processed for mRNA analysis following the manufacturer instructions. The total RNA of each sample was extracted with RNeasy mini kit (Qiagen), including a DNase step (RNase free DNase set, Qiagen, Barcelona, Spain).
|
| Label |
biotin
|
| Label protocol |
samples were labeled with GeneChip Terminal Labeling kit following protocol specifications
|
| |
|
| Hybridization protocol |
samples were hyb in Rat Gene Array 1.1 ST from Affymetrix, for 16 hours at 45 ºC and 60 rpm. Samples were washed and stained in the GeneTitan platform following manufacturer specifications.
|
| Scan protocol |
Arrays were scanned in the GeneTitan platform following manufacturer specifications
|
| Data processing |
CEL files obtained from scanner were normalized with RMA algorithm using R software and bioconductor associated packages.
|
| |
|
| Submission date |
May 15, 2013 |
| Last update date |
Oct 16, 2013 |
| Contact name |
Xavier Navarro |
| E-mail(s) |
xavier.navarro@uab.cat
|
| Organization name |
Univarsitat Autònoma de Barcelona
|
| Street address |
Edif. M, Campus UAB
|
| City |
Bellaterra |
| ZIP/Postal code |
E-08193 |
| Country |
Spain |
| |
|
| Platform ID |
GPL11534 |
| Series (1) |
| GSE46988 |
Expression data from rat spinal cord injury and mesenchymal stromal cells (MSC) or olfactory ensheathing cells (OEC) transplantation |
|
