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Status |
Public on Apr 02, 2015 |
Title |
PBM_P_Nb38 |
Sample type |
RNA |
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|
Source name |
peripheral blood monocytes, non-metastatic patient
|
Organism |
Homo sapiens |
Characteristics |
tumor stage: non-metastatic patient gender: F age: 54
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser, and quantification with Nanodrop.
|
Label |
Cy3
|
Label protocol |
Biotynilated cRNA was prepared with Ambion Illumina TotalPrep RNA Amplification Kit
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|
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Hybridization protocol |
Illumina Whole-Genome Gene Expression Direct Hybridization Assay
|
Scan protocol |
Standard Illumina Scanning protocol
|
Description |
5937302002_G
|
Data processing |
With GenomeStudio GX (version 1.5.4) we extracted from each array the background-corrected, summarized expression levels (AVG Signal), standard errors of the bead replicates (BEAD STERR), number of beads used (Avg NBEADS) and detection scores (Detection Pval). Subsequently, we applied a variance stabilizing transformation and a quantile normalization in R, as implemented in the lumi-package from Bioconductor.
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|
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Submission date |
Jun 07, 2013 |
Last update date |
Apr 02, 2015 |
Contact name |
Rekin's Janky |
E-mail(s) |
Nucleomics.Bioinformatics@vib.be
|
Organization name |
VIB
|
Department |
Nucleomics Core
|
Street address |
Herestraat 49 Box 816
|
City |
Leuven |
ZIP/Postal code |
B-3000 |
Country |
Belgium |
|
|
Platform ID |
GPL10558 |
Series (1) |
GSE47756 |
Tumor-educated circulating monocytes are powerful specific biomarkers for diagnosis of colorectal cancer |
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