|
| Status |
Public on Jun 30, 2006 |
| Title |
macrophages at 48 h post infection with B. melitensis strain 16M, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
murine macrophage J774.A1 cell line, from ATCC (#TIB-67)
|
| Organism |
Mus musculus |
| Characteristics |
infected with Brucella melitensis strain 16M for 48 hours
|
| Treatment protocol |
J774.A1 macrophages were infected with B. melitensis strain 16M
|
| Growth protocol |
J774.A1 macrophage cells were plated in 12 75-cm^2 flasks in c-DMEM without antibiotics 1 day prior to infection at a concentration of 8 x 10^6 cells per flask. Following 4h incubation at 37°C in an atmosphere containing 5% CO2, the cells were washed three times with PBS and treated with 50 ug/ml gentamicin to kill extracellular Brucella. The cells were collected for RNA isolation at 48 h post infection.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed, followed by Qiagen RNeasy Mini Kit cleanup according to the manufacturers' instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized on GeneChip mouse 430 2.0 arrays according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| Description |
Gene expression data from mouse J774.A1 macrophages infected with B. melitensis strain 16M for 48 hours.
|
| Data processing |
The data were analyzed with GCOS using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250.
|
| |
|
| Submission date |
Jun 30, 2006 |
| Last update date |
Aug 02, 2019 |
| Contact name |
Yongqun He |
| E-mail(s) |
yongqunh@umich.edu
|
| Phone |
734-615-8231
|
| Fax |
734-936-3235
|
| URL |
http://www.hegroup.org/
|
| Organization name |
University of Michigan
|
| Street address |
018 ARF, 1150 W. Medical Center Dr.
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE5202 |
Expression data from B. melitensis infected mouse macrophages |
|
| Relations |
| Reanalyzed by |
GSE119085 |
| Reanalyzed by |
GSE135324 |
