|
| Status |
Public on Aug 01, 2013 |
| Title |
IR_LGR5hi_ISC_4 |
| Sample type |
RNA |
| |
|
| Source name |
IR, LGR5hi, ISC
|
| Organism |
Mus musculus |
| Characteristics |
gender: female
genotype/variation: LGR5-GFPki
stress: irradiated
cell type: intestinal stem cells (ISCs)
facs-sortedcell type: LGR5hi
|
| Treatment protocol |
LGR5-GFPki mice were 12Gy gamma-irradiated or non-irradiated. LGR5hi and LGR5lo ISCs were FACS sorted 3 hours after irradiation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using the MagMAX-96 Viral RNA Isolation kit (Ambion) following manfacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
50ng of each sample was labeled with the Low Input Quick Amp Labeling Kit (Agilent Technologies) according to manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Cy3-labelled cRNA was fragmented and blocked following manufacturer's instructions.
|
| Scan protocol |
Slides were scanned using one color scan setting for GE 8x60K slides with the scanner (Agilent Technologies).
|
| Description |
gene expression irradiated
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 10.7.3.1 (Agilent). Further processing (background correction, normalization, summarization) was done following Agilent's quickstart guided workflow.
|
| |
|
| Submission date |
Aug 01, 2013 |
| Last update date |
Aug 01, 2013 |
| Contact name |
Hans A Kestler |
| E-mail(s) |
hans.kestler@uni-ulm.de
|
| Phone |
+49 731 5024248
|
| Organization name |
University of Ulm
|
| Department |
Research Group Bioinformatics and Systems Biology
|
| Street address |
Albert-Einstein-Allee 11
|
| City |
Ulm |
| ZIP/Postal code |
89081 |
| Country |
Germany |
| |
|
| Platform ID |
GPL10787 |
| Series (1) |
| GSE49461 |
Development of gene expression signatures for LGR5hi and LGR5lo ISCs in response to gamma-irradiation |
|
