|
| Status |
Public on Sep 04, 2013 |
| Title |
CD45.2+LSK_RUNX1S291fs |
| Sample type |
RNA |
| |
|
| Source name |
Bone marrow CD45.2+LSK cells, RUNX1S291fs, replicate 1
|
| Organism |
Mus musculus |
| Characteristics |
tissue: bone marrow LSKs
disease stage: normal, pre-MDS
genotype: RUNX1S291fs
cd45: CD45.2
cell type: Bone marrow CD45.2+LSK cells
genetic background: C57BL/6
|
| Treatment protocol |
Freshly isolated bone marrow cells were stained by appropriate antibodies, and then sorted on FACSAriaII (BD).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Plus Mini Kit (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
20 ng of total RNA was mixed with spike-in controls using an Agilent One Color Spike Mix Kit (Agilent), amplified and labeled with Cyanine 3 using a Low Input Quick Amp Labeling Kit (Agilent) according to the manufacturer's instructions, which generated single-color labeled cRNA.
|
| |
|
| Hybridization protocol |
600 ng of the labeled cRNA was hybridized to Agilent SurePrint G3 Mouse GE 8x60K Microarray. The process of hybridization and washing was performed using a Hi-RPM Gene Expression Hybridization Kit (Large) (Agilent) and a Gene Expression Wash Pack (Agilent), respectively.
|
| Scan protocol |
GeneChips were scanned using a DNA microarray scanner (Agilent Technologies)
|
| Description |
Gene expression at 4 months post-transplantation in RUNX1S291fs LSK cells
|
| Data processing |
Standard Agilent protocol
|
| |
|
| Submission date |
Sep 03, 2013 |
| Last update date |
Sep 05, 2013 |
| Contact name |
Goro Sashida |
| Organization name |
Kumamoto University
|
| Department |
IRCMS
|
| Lab |
Transcriptional Regulation in Leukemogenesis
|
| Street address |
2-2-1 Honjo, Chuo-ku
|
| City |
Kumamoto |
| State/province |
Kumamoto |
| ZIP/Postal code |
860-0811 |
| Country |
Japan |
| |
|
| Platform ID |
GPL10787 |
| Series (1) |
| GSE50537 |
Expression data from RUNX1S291fs-mutant and/or Ezh2 conditional knockout Lineage-c-Kit+Sca-1+ (LSK) cells |
|
