|
| Status |
Public on Dec 18, 2014 |
| Title |
SD1 - DOXY |
| Sample type |
RNA |
| |
|
| Source name |
SD-1 cells with doxycycline
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: SD-1
|
| Treatment protocol |
SD-1 Tet-On-Tight-HDAC7 or SD-1 cells were treated or not for 24 hours with doxycycline and RNA was collected
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized
|
| Scan protocol |
GeneChips were scanned using Gene Chip Operating Software (GCOS 1.2, Affymetrix)
|
| Description |
SD-1 cells with doxycycline
|
| Data processing |
The data were analyzed with Bioconductor affy package under statistical R software, computing the RMA (Robust Multichip Average) expression measure in log2 base scale described in Irizarry et al Biostatistics (2003).
|
| |
|
| Submission date |
Oct 30, 2013 |
| Last update date |
Dec 19, 2014 |
| Contact name |
Manel Esteller |
| Organization name |
IDIBELL
|
| Department |
PEBC
|
| Lab |
Cancer Epigenetics
|
| Street address |
Hospital Duran i Reynals Av. Gran Via s/n km, 2.7
|
| City |
L'Hospitalet de Llobregat |
| State/province |
Barcelona |
| ZIP/Postal code |
08908 |
| Country |
Spain |
| |
|
| Platform ID |
GPL13667 |
| Series (1) |
| GSE51895 |
Histone deacetylase 7 role in B cell acute lymphoblastic leukemia and B cell lymphoma |
|
