|
| Status |
Public on May 01, 2014 |
| Title |
NPTr_H1N1 (MGK 150) |
| Sample type |
RNA |
| |
|
| Source name |
NPTr_H1N1
|
| Organism |
Sus scrofa |
| Characteristics |
cell line: NPTr cells
infection: Influenza virus strain H1N1
|
| Treatment protocol |
NPTr cells were pre-infected 12 h with H1N1 at a MOI of 1. Where appropriate, NPTr cells were then infected with S. suis strain 31533 for 12 h at a MOI of 10
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from NPTr cells using QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Cyanine 3
|
| Label protocol |
cDNA was labelled with Cyanine 3 Labeling Master Mix according to Manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Standard Agilent hybridization protocol
|
| Scan protocol |
Standard Agilent scanning protocol
|
| Data processing |
The data were normalized with VSN method using Flexarray 1.6.2
|
| |
|
| Submission date |
Nov 07, 2013 |
| Last update date |
May 01, 2014 |
| Contact name |
Claude Lachance |
| E-mail(s) |
claude.lachance@mail.mcgill.ca
|
| Organization name |
Bio-Rad
|
| Street address |
1329 Meyerside Drive
|
| City |
Mississauga |
| State/province |
Ontario |
| ZIP/Postal code |
L5T 1C9 |
| Country |
Canada |
| |
|
| Platform ID |
GPL15007 |
| Series (1) |
| GSE52172 |
Transcriptional approach to study porcine tracheal epithelial cells individually or dually infected with swine influenza virus and Streptococcus suis |
|
