|
Status |
Public on May 01, 2014 |
Title |
NPTr_H1N1&S.suis (MGK 156) |
Sample type |
RNA |
|
|
Source name |
NPTr_H1N1&S.suis
|
Organism |
Sus scrofa |
Characteristics |
cell line: NPTr cells infection: Influenza virus strain H1N1 & Streptococcus suis strain 31533
|
Treatment protocol |
NPTr cells were pre-infected 12 h with H1N1 at a MOI of 1. Where appropriate, NPTr cells were then infected with S. suis strain 31533 for 12 h at a MOI of 10
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from NPTr cells using QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
Cyanine 3
|
Label protocol |
cDNA was labelled with Cyanine 3 Labeling Master Mix according to Manufacturer's protocol.
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|
|
Hybridization protocol |
Standard Agilent hybridization protocol
|
Scan protocol |
Standard Agilent scanning protocol
|
Data processing |
The data were normalized with VSN method using Flexarray 1.6.2
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|
|
Submission date |
Nov 07, 2013 |
Last update date |
May 01, 2014 |
Contact name |
Claude Lachance |
E-mail(s) |
claude.lachance@mail.mcgill.ca
|
Organization name |
Bio-Rad
|
Street address |
1329 Meyerside Drive
|
City |
Mississauga |
State/province |
Ontario |
ZIP/Postal code |
L5T 1C9 |
Country |
Canada |
|
|
Platform ID |
GPL15007 |
Series (1) |
GSE52172 |
Transcriptional approach to study porcine tracheal epithelial cells individually or dually infected with swine influenza virus and Streptococcus suis |
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