Healthy adult volunteers were randomized to be vaccinated subcutaneously with MPSV4 (Menomune®, n=13) or intramuscularly with MCV4 (MenactraTM, n=17) (Sanofi Pasteur, Swiftwater, PA), according to manufacture's recommendations.
Extracted molecule
total RNA
Extraction protocol
Total RNA was extracted from PBMC using Trizol (Invitrogen, 1 ml of Trizol to ~1.5 million cells) according to manufacture's recommendation.
Label
biotin
Label protocol
Per Affymetrix protocol
Hybridization protocol
Per Affymetrix protocol
Scan protocol
Per Affymetrix protocol
Data processing
Hybridization data of all samples passed quality control and expression data were normalized by RMA R package
