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Sample GSM1266601 Query DataSets for GSM1266601
Status Public on Jan 01, 2014
Title Prep1deltaSM_P70
Sample type RNA
 
Source name tibialis anterior muscle, Prep1deltaSM
Organism Mus musculus
Characteristics strain/background: C57BL/6
genotype/variation: Prep1deltaSM
gender: male
age: 8 weeks
tissue: tibialis anterior muscle
Extracted molecule total RNA
Extraction protocol RNA was prepared using TRIzol reagent according to the manufacturer's instruction. RNA was subjected to DNase digestion to remove genomic DNA. Quantification of RNA was performed on a Nanodrop-1000 and RNA integrity was measured on Agilents Bioanalyzer.
Label Cy3
Label protocol Cy3-labeled cRNA was prepared from 1 ug RNA using the One-Color Low RNA Input Linear Amplification PLUS kit from Agilent according to the manufacturer's instructions, followed by RNeasy column purification. Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.
 
Hybridization protocol 1.5 ug of Cy3-labelled cRNA (specific activity >10.0 pmol Cy3/ug cRNA) was fragmented at 60°C for 30 minutes in a reaction volume of 250 ml containing 1x Agilent fragmentation buffer and 2x Agilent blocking agent following the manufacturer's instructions. On completion of the fragmentation reaction, 250 ml of 2x Agilent hybridization buffer was added to the fragmentation mixture and hybridized to Agilent-014868 Whole Mouse Genome Oligo Microarrays (G4112A) for 17 hours at 65°C in a rotating Agilent hybridization oven. After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent) and 1 minute with 37°C GE Wash buffer 2 (Agilent), then dried immediately by brief centrifugation.
Scan protocol Slides were scanned using one color scan setting for 4x44k array slides.
Description P70
RNA from tibialis muscle of Prep1deltaSM mice.
Data processing The scanned images were analyzed with Feature Extraction Software 9.1 (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities.
 
Submission date Nov 15, 2013
Last update date Jan 01, 2014
Contact name Timo Kanzleiter
Organization name DIfE
Department Experimental Diabetology
Street address Arthur-Scheunert-Alle 114
City Nuthetal
ZIP/Postal code 14558
Country Germany
 
Platform ID GPL7202
Series (1)
GSE52424 Gene expression from Prep1-ablated mice

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
A_51_P518959 61.78993875
A_52_P221057 17.33656125
A_52_P128999 16.55296875
A_51_P101283 27.6602575
A_51_P103133 3.026388375
A_51_P104977 3.286592875
A_51_P107701 1.46588725
A_51_P111434 1.7991475
A_51_P112476 1.488428375
A_51_P114236 460.118075
A_51_P114348 1.2997575
A_51_P114403 1.51192525
A_51_P114407 3879.26575
A_51_P115192 1.474969625
A_51_P116157 73.65156625
A_51_P116226 1.494353625
A_51_P123724 349.5774875
A_51_P132944 5.28630625
A_51_P133148 3.494239875
A_51_P133953 930.934475

Total number of rows: 32812

Table truncated, full table size 774 Kbytes.




Supplementary file Size Download File type/resource
GSM1266601_P70.txt.gz 8.5 Mb (ftp)(http) TXT
Processed data included within Sample table

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