|
| Status |
Public on Aug 18, 2014 |
| Title |
Ad Libitum 7 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse epididymal fat
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL6/J
age: 8 months
tissue: Liver
Sex: Male
treatment: Ad Libitum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from 25 mg of liver in each sample using Qiagen Rneasy kit following manufacturer’s protocol. RNA quantity was assessed by Nanodrop and RNA quality was assessed by Agilent Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using Ambion kit- Illumina TotalPrep RNA Amplification Kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
AL07
|
| Data processing |
Data was processed in R by log2 transformation and z-normalization
|
| |
|
| Submission date |
Nov 29, 2013 |
| Last update date |
Aug 18, 2014 |
| Contact name |
Wilson Chun Yim Fok |
| E-mail(s) |
wilson.fok@wustl.edu
|
| Organization name |
Washington University in St. Louis
|
| Department |
Hematology
|
| Street address |
660 S Euclid Ave
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE52825 |
Short-term rapamycin treatment in mice have few effects on the transcriptome of white adipose tissue compared to dietary restriction |
|
