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Sample GSM1277671 Query DataSets for GSM1277671
Status Public on Nov 24, 2014
Title SOD 155 -/- (2)
Sample type RNA
 
Source name Lumbar spinal cord homogenate
Organism Mus musculus
Characteristics cell type: Lumbar spinal cord homogenate
strain/genotype: Mouse -B6/SJL-mSOD1G93A-miR-155-/-
gender: female
Treatment protocol NA
Growth protocol NA
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using mirVanaTM miRNA isolation kit (Ambion) according to the manufacturer’s protocol
Label NA
Label protocol nCounter Mouse miRNA Assay Kit
 
Hybridization protocol according to the manufacturer’s protocol
Scan protocol according to the manufacturer’s protocol
Data processing Data was proccessed using nSolver, normalized to Positive controls, Housekeeping genes and negative controls
 
Submission date Dec 02, 2013
Last update date Nov 24, 2014
Contact name Oleg Butovsky
E-mail(s) obutovsky@rics.bwh.harvard.edu
Phone 1-617-525-5313
Organization name Brigham and Women's Hospital, Harvard Medical School
Department Center of Neurologic Diseases
Lab Dr. Oleg Butovsky
Street address 77 Avenue Louis Pasteur, Office 614
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL18014
Series (2)
GSE52898 miRNA profile in lumbar spinal cord homogenate of SOD-miR155 Tg mice
GSE52947 Targeting miR-155 restores abnormal microglia and attenuates disease in SOD1 mice

Data table header descriptions
ID_REF
VALUE Normalized mRNA transcripts copies per 100ng of total RNA

Data table
ID_REF VALUE
mmu-let-7g 2503
mmu-let-7i 984
mmu-miR-1 15
mmu-miR-15b 242
mmu-miR-23b 359
mmu-miR-27b 45
mmu-miR-29b 4017
mmu-miR-30a 54
mmu-miR-30b 150
mmu-miR-99a 944
mmu-miR-99b 74
mmu-miR-101a 29
mmu-miR-124 3044
mmu-miR-125a-5p 2266
mmu-miR-125b-5p 6744
mmu-miR-126-5p 47
mmu-miR-126-3p 2207
mmu-miR-127 190
mmu-miR-128 83
mmu-miR-130a 51

Total number of rows: 599

Table truncated, full table size 9 Kbytes.




Supplementary file Size Download File type/resource
GSM1277671_20121129_sod_155_lumbar_mirna_01_02.RCC.gz 5.2 Kb (ftp)(http) RCC
Processed data included within Sample table

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