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Sample GSM1309418 Query DataSets for GSM1309418
Status Public on Jan 18, 2014
Title undifferentiated H1 hESC biological rep 2
Sample type RNA
 
Source name H1 hESC undifferentiated
Organism Homo sapiens
Characteristics cell line: H1
cell type: human embryonic stem cells (hESCs)
cell status: undifferentiated
Growth protocol hESCs were cultured in mTeSR1 medium for undifferentiated cells. For EB formation, cells were dissociated with Accutase (Stem Cell Technologies, Vancouver, Canada) and plated on ultra low attachment plates (Corning Life Science, Tewksbury, MA) in hESGM (without bFGF) with 10 μM Y-27632 (Chemdea, Ridgewood, NJ) for designated period of time.
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridazation was performed according to standard protocol recommended by Affymetrix.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description H1_und_2
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Jan 17, 2014
Last update date Jan 18, 2014
Contact name Yong Kim
E-mail(s) thadyk@ucla.edu
Organization name UCLA
Street address 10833 Le Conte Ave, 73-022 CHS
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
 
Platform ID GPL570
Series (1)
GSE54186 Discovery of consensus gene signature and intermodular connectivity defining self-renewal of human embryonic stem cells

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
1007_s_at 2199.897
1053_at 1642.938
117_at 40.66708
121_at 618.038
1255_g_at 857.3466
1294_at 63.29601
1316_at 80.13531
1320_at 56.39448
1405_i_at 0.7402939
1431_at 26.56045
1438_at 44.76184
1487_at 626.512
1494_f_at 110.3081
1552256_a_at 1124.785
1552257_a_at 2555.838
1552258_at 55.7356
1552261_at 32.80069
1552263_at 816.7018
1552264_a_at 561.2803
1552266_at 12.40542

Total number of rows: 54675

Table truncated, full table size 1052 Kbytes.




Supplementary file Size Download File type/resource
GSM1309418_H1_und_2.CEL.gz 4.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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