|
| Status |
Public on Feb 14, 2014 |
| Title |
GANP KD Cytoplasmic RNA Experiment 3 |
| Sample type |
RNA |
| |
|
| Source name |
HCT116 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HCT116
transfection: GANP siRNA
|
| Treatment protocol |
Cells were transfected with Control siRNA, GANP siRNA or NXF1 siRNA and cytoplasmic RNA was harvested 72 hours post-transfection.
|
| Extracted molecule |
cytoplasmic RNA |
| Extraction protocol |
RNA was extracted from cytoplasmic fraction of cells (obtained by digitonin permeabilisation) with QIAGEN RNeasy mini kit according to manufacturers instructions. RNA was treated with RNase free DNase (Qiagen) prior to elution. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared with the Illumina 96-well Total Prep RNA Amplification Kit (Ambion)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
The data were normalised using quantile normalisation with R package lumi
|
| |
|
| Submission date |
Jan 28, 2014 |
| Last update date |
Feb 14, 2014 |
| Contact name |
Vihandha Wickramasinghe |
| Organization name |
MRC Cancer Unit, Hutchison/MRC Research Centre
|
| Lab |
Venkitaraman
|
| Street address |
Hills Road
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0XZ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6884 |
| Series (1) |
| GSE54481 |
Selective nuclear export of specific classes of mRNA from mammalian nuclei is promoted by GANP |
|
