|
| Status |
Public on Jul 02, 2014 |
| Title |
leukocytes_smoker_old_male_0min_rep4 |
| Sample type |
RNA |
| |
|
| Source name |
Total leukocytes
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
cell type: leukocyte
age: >50 y.o.
smoking status: Smoker
disease status: Healthy
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Erythrocytes were lysed from whole blood, total leukocytes were washed twice and total RNA was extracted.
Total RNA was extracted using QIAamp RNA Blood Mini Kit.
|
| Label |
biotin
|
| Label protocol |
Sample preparation for microarray hybridization was performed following Affymetrix's protocols. The GeneChip 3'IVT Express Protocol was used for sample labeling. Briefly, from 150 ng total RNA a biotin-labeled cRNA was generated by reverse transcription, followed by an IVT.
|
| |
|
| Hybridization protocol |
Following cRNA fragmentation, the sample was hybridized on the GeneChip HT HG-U219 Array Plate.
|
| Scan protocol |
Hybridization, washes and scanning were processed in the Gene Titan instrument, a fully automated array system.
|
| Description |
* This sample has not been included in related publications, detected as outlier
$ This sample has not been analyzed in the first publication.
|
| Data processing |
Raw expression measures were summarized after background correction and normalization steps using the rma methodology in the Expression Console™ Software 1.2.1.20. Batch effect (by plates) was corrected using Partek® Software
|
| |
|
| Submission date |
Mar 17, 2014 |
| Last update date |
Jul 02, 2014 |
| Contact name |
Susana Kalko |
| E-mail(s) |
kalko.susana@gmail.com
|
| Organization name |
IDIBAPS
|
| Department |
Bioinformatics Platform
|
| Street address |
c/Rosello 153
|
| City |
Barcelona |
| State/province |
BARCELONA |
| ZIP/Postal code |
08036 |
| Country |
Spain |
| |
|
| Platform ID |
GPL13667 |
| Series (1) |
| GSE55962 |
Systemic inflammatory response to smoking in chronic obstructive pulmonary disease: evidence of a gender effect |
|
