|
| Status |
Public on Mar 26, 2014 |
| Title |
Axenic flies, biological replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Flies at 7 days of age raised and kept axenic after emergence
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: whole flies
genotype: yellow white
treatment: raised and kept axenic after emergence
|
| Extracted molecule |
total RNA |
| Extraction protocol |
NucleoSpin RNA clean up from, Macherey Nagel, as described in details in the related article that will be published soon in PLoS One
|
| Label |
biotin
|
| Label protocol |
One microgram of total RNA was amplified according Affymetrix protocol (IVT), to generate a minimum of 90 g of biotinylated cRNA
|
| |
|
| Hybridization protocol |
Hybridization was conducted as recommended by Affymetrix using dedicated systems and protocol using 7.5 g biotinilated cRNA by chip
|
| Scan protocol |
Affymetrix microarrays were scanned using a GCS3000 scanner
|
| Description |
Gene expression data from flies at 7 days of age raised and kept axenic after emergence
|
| Data processing |
Raw data were processed using Affymetrix Command Console to generate CEL files
|
| |
|
| Submission date |
Mar 25, 2014 |
| Last update date |
Mar 26, 2014 |
| Contact name |
François Leulier |
| E-mail(s) |
francois.leulier@ens-lyon.fr
|
| Organization name |
Institut of Functional Genomics Lyon
|
| Street address |
46, allée d'Italie
|
| City |
Lyon |
| ZIP/Postal code |
69364 |
| Country |
France |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE56173 |
Expression data from Adult Drosophila with controled microbiota |
|
