|
| Status |
Public on Mar 28, 2015 |
| Title |
One high dose of 17beta-estradiol_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Ventral prostate, one high dose of 17beta-estradiol, three days
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Wistar
tissue: Ventral prostate
gender: Male
age: Adult- 90 days old
protocol: Estrogen
|
| Treatment protocol |
On the 90th day after birth, the rats were assigned to different treatment groups (n=3): First group were castrated (Cas) by orchiectomy via scrotal incision under ketamine (150 mg/Kg body weight) and xylazin (10 mg/kg body weight) anesthesia; Second group received a 25 mg/Kg body weight dose of 17β-estradiol diluted in corn oil (E2 group);Third group received a combination of both treatments (Cas+E2 group) (combined orchiectomy and 17β-estradiol). In the control group (Ct; normal androgen and estrogen), the animals received only the vehicle.
|
| Growth protocol |
Male Wistar rats were kept under normal light conditions (12-h light:dark cycle) and received filtered tap water and Purina rodent chow ad libitum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ventral prostates were dissected under RNAse-free conditions. Thirty mg of the tissue was used for total RNA extraction. Subsequently, the tissue fragments were extracted using Illustra RNAspin Mini kits (GE Healthcare, Buckinghamshire,UK) according to the manufacturer’s instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated single-straded cDNA were prepared according to the standard Affymetrix protocol from 500ng total RNA (GeneChip® WT Terminal Labeling and Hybridization User Manual for use with the Ambion® WT Expression Kit, 2009, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 6.75 ug (27 ul at 25ng/ul) of fragmented and labeled single-straded cDNA were hibridized for 18 hrin 45°C hybridization oven, at 60 rpm. GeneChips were washed and stained in the GeneChip Fluidics Station 450 (Affymetrix).
|
| Scan protocol |
GeneChip were scanned Affymetrix GeneChip Operating Software v1.3 program.
|
| Description |
Gene expression data from ventral prostate colected from adult rats three days after received a 25 mg/Kg body weight dose of 17β-estradiol diluted in corn oil
|
| Data processing |
The data were summarized and normalized by Expression Console software (Affymetrix).
|
| |
|
| Submission date |
Mar 27, 2014 |
| Last update date |
Mar 28, 2015 |
| Contact name |
Rafaela da Rosa Ribeiro |
| E-mail(s) |
rafaela.darosa@hotmail.com
|
| Organization name |
State University of Campinas
|
| Department |
Structural and functional biology department
|
| Street address |
Charles Dawin st, bl.N room 10
|
| City |
Campinas |
| ZIP/Postal code |
13083-863 |
| Country |
Brazil |
| |
|
| Platform ID |
GPL6247 |
| Series (1) |
| GSE56289 |
Rat ventral prostate gland gene profile after androgen deprivation |
|
