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Status |
Public on Oct 29, 2014 |
Title |
048_Ps_302_CD14-_wk10 |
Sample type |
RNA |
|
|
Source name |
048_Ps_302_CD14-_wk10
|
Organism |
Homo sapiens |
Characteristics |
Sex: F age: 24 cell type: primary cell cell subtype: CD14- disease status: diseased disease: Psoriasis time point: wk10
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from CD14- cells using the RNeasy Mini Kit with DNase digestion on the column (Qiagen, Inc. Valencia, CA, USA) as per the manufacturer’s instructions.
|
Label |
Biotin
|
Label protocol |
Target was prepared from 50ng total RNA using NuGEN Ovation V2 kits, followed by NuGEN Encore biotin labeling module using the manufacturer's protocol
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|
|
Hybridization protocol |
All samples were hybridized in parallel to different arrays on the same HT array plate using the manufacturer's protocol. Arrays were washed and stained on an Affymetrix GeneTitan instrument
|
Scan protocol |
Arrays were scanned using an Affymetrix GeneTitan MC scanner
|
Data processing |
Affymetrix .cel files were RMA normalized in ArrayStudio from Omicsoft Inc (version 4.1). Differential gene expression was analyzed using paired t-tests and genes were selected based on p-value (affymetrix probe sets with no associated gene annotation were not included)
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Submission date |
May 07, 2014 |
Last update date |
Oct 29, 2014 |
Contact name |
Christopher Ritchlin |
E-mail(s) |
Christopher_Ritchlin@URMC.Rochester.edu
|
Phone |
(585) 486-0901
|
Organization name |
University of Rochester Medical Center
|
Department |
School of Medicine and Dentistry
|
Street address |
601 Elmwood Ave, BOX695
|
City |
Rochester |
State/province |
NY |
ZIP/Postal code |
14642 |
Country |
USA |
|
|
Platform ID |
GPL13158 |
Series (2) |
GSE57386 |
Gene expression of biopsies, CD14+ and CD14- cells from RA, PsA and PsO patients with Infliximab treatment |
GSE57405 |
Gene expression of CD14- cells from RA, PsA and PsO patients with Infliximab treatment |
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