|
| Status |
Public on Jul 01, 2007 |
| Title |
Differential gene expression in BALB/c Npc1-/- and Npc1+/+ cerebellum - animal No 12 |
| Sample type |
RNA |
| |
|
| Source name |
BALB/c Npc1-/- and Npc1+/+ cerebellum
|
| Organism |
Mus musculus |
| Characteristics |
Strain: BALB/c Npc1
Tissue: cerebellum
Time point: P49
Genotype: Npc1-/-
Gender: female
|
| Biomaterial provider |
Jackson laboratories, Bar Harbor (ME)
|
| Treatment protocol |
Npc1 genotype was determined by PCR
Sacrified by cervical extension without anaesthetics at P21
Cerebellum was removed and tissue stored in RNAlater RNA stabilization reagent (Qiagen, Hilden, Germany) at –80 centigrade
|
| Growth protocol |
Housing conditions: 12 hours light/dark cycle
Standard chow diet
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The tissue was minced in liquid nitrogen using pestle and mortar. Then, the TRIZOL (Life technologies, Karlsruhe, Germany) was used followed by the RNeasy Mini Kit procedure (Qiagen) including DNAse treatment. Quality and quantity of the extract was checked with RNA 6000 Pico LabChip (Agilent).
|
| Label |
biotin
|
| Label protocol |
according to Affymetrix
|
| |
|
| Hybridization protocol |
according to Affymetrix
|
| Scan protocol |
according to Affymetrix
|
| Description |
Replicate 3 of 3 (same genotype, same age). All replicates were individual animals.
|
| Data processing |
Arrays were scanned twice with GeneChip Scanner 3000 (Affymetrix) and analysed with GeneChip Operating Software 1.3 (Affymetrix). Average hybridisation intensity was set to 500 per gene.
|
| |
|
| Submission date |
Sep 29, 2006 |
| Last update date |
Oct 29, 2008 |
| Contact name |
Roland Distl |
| E-mail(s) |
roland.distl@web.de
|
| Phone |
+49-30-450-536004
|
| Organization name |
Charité Universitätsmedizin Berlin
|
| Department |
Institute of Neuropathology
|
| Street address |
Am Augustenburger Platz 1
|
| City |
Berlin |
| ZIP/Postal code |
13353 |
| Country |
Germany |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
| GSE5944 |
Differential gene expression in BALB/c Npc1-/- and Npc1+/+ cerebellum |
|
