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Status |
Public on Dec 31, 2015 |
Title |
siRNA against Sat III transfection, heat shock treatment followed by recovery, replicate 1 |
Sample type |
RNA |
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Source name |
HEK293T cells, siRNA against Sat III, heat shock followed by recovery
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Organism |
Homo sapiens |
Characteristics |
cell line: HEK293T sirna: against Sat III treatment: heat shock followed by recovery
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Treatment protocol |
Heat shocks were performed for 1 h at 42 °C by submersion in a water bath. Recovery was for 6 h.
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Growth protocol |
Human HEK293T cells were cultured in Dulbecco's Modified Eagle's medium. The media were supplemented with 10% fetal calf serum, 2 mM L-glutamine and antibiotics (penicillin and streptomycin), and the cells were cultured in a humidified 5% CO2 atmosphere at 37°C.
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from HEK293T cells using the RNeasy kit (Qiagen).
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Label |
Biotin
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Label protocol |
To synthesize the biotinylated cRNA, 300 ng of total RNA was amplified using Ambion's Illumina TotalPrep RNA Amplification kit (cat #AMIL1791).
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Hybridization protocol |
From each sample, 750 ng cRNA was hybridized to Illumina's HumanHT-12 Expression BeadChip, version 3 (cat no BD-103-0203) at 58°C overnight (19 h) according to Illumina Whole-Genome Gene Expression Direct Hybridization protocol, revision A.
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Scan protocol |
The chips were scanned with Illumina BeadArray Reader, BeadScan software version 3.5, and numerical results were extracted with Illumina's GenomeStudio v1.0 software without any normalization.
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Description |
siRNA III 1h HS 6hR 1 Replicate 1
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Data processing |
The gene expression microarray data was analyzed using R and Bioconductor softwares. The data was quantile normalized. The Sample data table and non-normalized data matrix do not include values for the control probes.
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Submission date |
Jun 12, 2014 |
Last update date |
Dec 31, 2015 |
Contact name |
Lea Sistonen |
Organization name |
Åbo Akademi
|
Department |
Department of Biosciences
|
Street address |
Tykistökatu 6 B
|
City |
Turku |
ZIP/Postal code |
20520 |
Country |
Finland |
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Platform ID |
GPL6947 |
Series (1) |
GSE58439 |
Long non-coding Satellite III RNAs link the heat shock response and cell proliferation |
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