|
| Status |
Public on Jul 01, 2014 |
| Title |
wild-type WKMs, biological rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
zebrafish kidney
|
| Organism |
Danio rerio |
| Characteristics |
tissue: kidney
cell type: WKMs(without erythrocytes) sorted from kidney
genotype: wild type
age: 60 dpf
|
| Treatment protocol |
The kidney was dissected from 60 dpf zebrafish on ice, rinsed with 0.9 x PBS and 10% FBS, and then passed through the 40 μm filter. Myelomonocytes or WKMs (without erythrocytes) were sorted from the single cell suspension.
|
| Growth protocol |
Zebrafish embryos were raised up in standard conditions (as described in The Zebrafish Book).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200 ng total RNA (GeneAtlas® WT Expression User Manual, 2012, Affymetrix).
|
| |
|
| Hybridization protocol |
10μg fragmented, labeled cRNA were hybridized for 16 hours at 45℃ on Affymetrix Zebrafish 1.0 ST array.
|
| Scan protocol |
GeneChips were scanned with GeneChip® Scanner 3000.
|
| Description |
Gene expression data from WKMs(without erythrocytes) in zebrafish kidney
|
| Data processing |
The data were analyzed according to the GeneChip® Expression Analysis Manual (Affymetrix).
|
| |
|
| Submission date |
Jun 30, 2014 |
| Last update date |
Jul 01, 2014 |
| Contact name |
Hongbo Fan |
| E-mail(s) |
fanhongbo86@126.com
|
| Organization name |
Chinese Academy of Sciences
|
| Department |
Institute of Health Sciences
|
| Street address |
320 Yueyang Road, Shanghai, China
|
| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
| |
|
| Platform ID |
GPL16933 |
| Series (1) |
| GSE58944 |
Gene expression data from myelomonocytes and whole kidney marrows in zebrafish adult kidney after deletion of miR-142-3p |
|
