|
| Status |
Public on Jan 30, 2015 |
| Title |
backcross mouse ID 17335 |
| Sample type |
RNA |
| |
|
| Source name |
(129xFVBN) F1 x129 backcross mouse
|
| Organism |
Mus musculus |
| Characteristics |
Sex: 0
tumor: 0
age (days): 401
|
| Growth protocol |
Superior Cervical Ganglia were dissected from backcrossed mice at time of tumor harvest or 1 year of age
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted and purified using the Rneasy mini kit
|
| Label |
biotin
|
| Label protocol |
Labeling was performed according to the Affymetrix (Santa Clara, CA) GeneChip Whole Transcript (WT) Sense Target Labeling Assay manual using kit 900652.
|
| |
|
| Hybridization protocol |
200 ul of hybridization cocktail were injected into microarrays and hybridized at 45°C for 16 hr at 60 rpm. Arrays were washed with fluidics station protocol: FS450_0001
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000 7G
|
| Description |
pooled pair of SCG from single backcross mouse
|
| Data processing |
Expression was based on RMA values assessed using the Bioconductor XPS package in R on only core probes
probe group file: MoEx-1_0-st-v1.r2.pgf
meta-probeset file: MoEx-1_0-st-v1.r2.dt1.mm9.core.mps
|
| |
|
| Submission date |
Jul 22, 2014 |
| Last update date |
Jan 30, 2015 |
| Contact name |
William A. Weiss |
| Organization name |
University of California, San Francisco
|
| Department |
Neurology
|
| Lab |
William A. Weiss
|
| Street address |
1450 3rd Street, HD-220
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platform ID |
GPL6096 |
| Series (1) |
| GSE59675 |
Expression analysis of superior cervical ganglion from backcrossed TH-MYCN transgenic mice |
|
