|
| Status |
Public on Mar 26, 2015 |
| Title |
IFNg_LPS rep2 |
| Sample type |
RNA |
| |
|
| Source name |
BMDM_IFNg_LPS
|
| Organism |
Mus musculus |
| Characteristics |
strain: Specific pathogen-free C57Bl/6
cell type: bone marrow-derived macrophage (BMDM)
stimulated: IFNγ for 18hrs and LPS for 6hrs
|
| Treatment protocol |
BMDMs were stimulated with 10ng/ul ultrapure Ecoli lipopolysaccharide (LPS).
|
| Growth protocol |
Specific pathogen-free C57Bl/6 mice were purchased from Harlan Sprague-Dawley (Horst, the Netherlands) and housed in a controlled environemt on 12h/12h light/dark cycles.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy kit procedures including DNAse digestion.
|
| Label |
biotin
|
| Label protocol |
standard biotin Ambion labeling - Illumina
|
| |
|
| Hybridization protocol |
standard Illumina protocol
|
| Scan protocol |
standard Illumina protocol
|
| Description |
7165861070_E
|
| Data processing |
Estimated background was subtracted from the foreground for each bead. For replicate beads, outliers greater than 3 median absolute deviations from the median were removed and the average signal was calculated for the remaining intensities. For each probe a detection score was calculated by comparing its average signal with the summarized values for the negative control probes. Non-expressed probes were removed yielding 15978 expressed probes (detection p < 0.05). Expressed probe data were neqc normalized.
|
| |
|
| Submission date |
Aug 11, 2014 |
| Last update date |
Mar 26, 2015 |
| Contact name |
Brendon Scicluna |
| E-mail(s) |
brendon.scicluna@um.edu.mt
|
| Organization name |
University of Malta
|
| Street address |
Msida campus
|
| City |
Msida |
| ZIP/Postal code |
MSD2020 |
| Country |
Malta |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE60290 |
Genome-wide analysis of bone marrow-derived macrophage (BMDM) priming by Ifng and Ifnb. |
|
