|
| Status |
Public on Aug 13, 2014 |
| Title |
Non-Responders to the Novel Mitochonrial Inhibitor CPI-613 rep3 |
| Sample type |
RNA |
| |
|
| Source name |
human peripheral blood mononuclear cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: blood mononuclear cells
age: 80
diagnosis: AML
gender: M
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from peripheral blood mononuclear cells using the RiboPure-Blood Kit (Life Technologies (AM1928)).
|
| Label |
Biotin
|
| Label protocol |
250 ng total RNA was amplified and labeled according to the Affymetrix 3’-IVT labeling protocol.
|
| |
|
| Hybridization protocol |
10 ug biotinylated cRNA was hybridized to the Affymetrix Gene Atlas U219 Human Genome array strips according to current Affymetrix protocols.
|
| Scan protocol |
The arrays were washed and scanned according to standard Affymetrix GeneAtlas instructions using the GeneAtlas scanner and software control(GeneAtlas Instrument Control (GAIC) v. 1.0.6.).
|
| Data processing |
Raw data (CEL files) were generated using Affymetrix AGCC software and quantile normalized by the RMA method. Log intensity distributions, pair-wise correlations and internal Affymetrix controls were examined to evaluate hybridization quality using Partek Genomics Suite software (Partek Inc.).
|
| |
|
| Submission date |
Aug 12, 2014 |
| Last update date |
Aug 13, 2014 |
| Contact name |
Jeff Chou |
| E-mail(s) |
jchou@wakehealth.edu
|
| Phone |
3367572617
|
| Organization name |
Atrium Health Wake Forest Baptist
|
| Street address |
Medical Center Blvd
|
| City |
Winston-Salem |
| State/province |
NC |
| ZIP/Postal code |
27157 |
| Country |
USA |
| |
|
| Platform ID |
GPL13667 |
| Series (1) |
| GSE60350 |
Expression Profiles of Responders versus Non-Responders with AML or MDS to the Novel Mitochondrial Inhibitor CPI-613 |
|
