Grown in AIM V with 2% Human AB Serum, 37degC, 5% CO2
Extracted molecule
total RNA
Extraction protocol
Agilent RNA isolation mini-kit
Label
Cy3
Label protocol
Agilent Low RNA Input Fluorescent Linear Amplification kit (v2.0) (reverse transcription with oligo-dT primers, in vitro transcription with direct incorporation of fluorescently labeled CTP analogs)
Agilent Low RNA Input Fluorescent Linear Amplification kit (v2.0) (reverse transcription with oligo-dT primers, in vitro transcription with direct incorporation of fluorescently labeled CTP analogs)
Hybridization protocol
Followed Agilent 60-mer oligo microarray processing protocol v 4.1, using SSC wash protocol and forced nitrogen drying
Scan protocol
Scanned using Agilent Microarray Scanner (G2565BA) at 10um resolution
Description
primary T cell response to oxidative stress under the context of T-cell activaiton
Data processing
Agilent Feature Extraction software (v.7.2) was used to assess spot quality and extract feature intensity statistics; duplicate spots were averaged; background-subtracted data were normalized using SNNLERM (Yang et al., PNAS 2003; 100:1122-1127)