|
| Status |
Public on Jun 30, 2016 |
| Title |
Female gonad_11m |
| Sample type |
RNA |
| |
|
| Source name |
Female gonad
|
| Organism |
Acipenser schrenckii |
| Characteristics |
gender: Female
tissue: gonad
condition: normal
age: 11 months
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using miRNeasy Kit(Qiagen, 217004), following the manufacturer's recommended protocol.
|
| Label |
Cy3
|
| Label protocol |
The assay started from 2 to 5 ug total RNA sample, which was size fractionated using a YM-100 Microcon centrifugal filter (from Millipore) and the small RNAs (< 300 nt) isolated were 3'-extended with a poly(A) tail using poly(A) polymerase. An oligonucleotide tag was then ligated to the poly(A) tail for later fluorescent dye staining; two different tags (Cy3/Cy5) were used for the two RNA samples in dual-sample experiments.
|
| |
|
| Hybridization protocol |
Hybridization was performed overnight on a µParaflo microfluidic chip using a micro-circulation pump (Atactic Technologies).
|
| Scan protocol |
Hybridization images were collected using a laser scanner (GenePix 4000B, Molecular Device) and digitized using Array-Pro image analysis software (Media Cybernetics).
|
| Description |
earlier sex differentiation
|
| Data processing |
Data were analyzed by first subtracting the background and then normalizing the signals using a LOWESS filter (Locally-weighted Regression). For two color experiments, the ratio of the two sets of detected signals (log2 transformed, balanced) and p-values of the t-test were calculated; differentially detected signals were those with less than 0.01 p-values.
|
| |
|
| Submission date |
Oct 28, 2014 |
| Last update date |
Jun 30, 2016 |
| Contact name |
Zhang Xiujuan |
| E-mail(s) |
zhangxj67@gdei.gd.cn
|
| Phone |
020-89100920
|
| Organization name |
Guangdong Entomological Institute
|
| Lab |
Guangdong Public Laboratory of Wild Animal Conservation and Utilization
|
| Street address |
105 Xingang West Road, Haizhu District
|
| City |
Guangzhou City |
| State/province |
Guangdong Province |
| ZIP/Postal code |
510260 |
| Country |
China |
| |
|
| Platform ID |
GPL19362 |
| Series (1) |
| GSE62787 |
Sex-biased expression of miRNAs by microarray in critically endangered Amur sturgeon |
|
