|
| Status |
Public on Apr 12, 2015 |
| Title |
3.NB4-DMSO |
| Sample type |
RNA |
| |
|
| Source name |
NB4 cell line
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: acute promyelocytic leukemia (APL)
cell line: NB4
treatment: treated with DMSO
|
| Treatment protocol |
NB4 cells were treated with 10 uM ATRA (Sigma Aldrich) or doxycycline-induced Pin1 knockdown for 3 days. When harvesting, cells were washed with Hank's Balanced Salt Solution(HBSS) (Invitrogen) and placed on ice in the Trizol solution (GibcoBRL).
|
| Growth protocol |
NB4 cells were cultured in RPMI1640 mudium with 10% fetal bovine serum (FBS) and all of the cells were cultured at 37°C in a humidified incubator containing 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Target preparation performed using Affymetrix (Technical Manual) WT Plus reagent kit, including labeling
|
| |
|
| Hybridization protocol |
Amplified, fragmented, and biotin labeled single-stranded cDNA (ss-cDNA) material was hybridized in Affymetrix GeneChip Mouse Transcription Assay 1.0
|
| Scan protocol |
Hybridized arrays were proceeding with Affymetrix GeneChip Hybridization Wash and Stain kit and then scanned.
|
| Description |
NB4 cells treated with DMSO
|
| Data processing |
The data were analyzed with Affymetrix ExpressionConsole and TranscriptomeAnalysisConsole using Affymetrix default analysis settings
|
| |
|
| Submission date |
Nov 06, 2014 |
| Last update date |
Apr 12, 2015 |
| Contact name |
Kun Ping Lu |
| Organization name |
Beth Israel Deaconess Medical Center
|
| Street address |
330 Brookline Ave
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE63059 |
Expression data from Pin1 inhibitor ATRA and Pin1 knockdown |
|
