|
| Status |
Public on Jun 18, 2016 |
| Title |
5% protein 1wk G121_C09_rat50 |
| Sample type |
RNA |
| |
|
| Source name |
Liver
|
| Organism |
Rattus norvegicus |
| Characteristics |
treatment: 5% protein 1wk
gender: Male
strain: Wistar
tissue: liver
|
| Treatment protocol |
RNA was extracted out the liver after 1wk or 4wks feeding an dieet with 5 or 20% protein
|
| Growth protocol |
RNA was extracted out the liver after 1wk or 4wks feeding an dieet with 5 or 20% protein
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA
|
| Label |
Biotin
|
| Label protocol |
The Affymetrix GeneChip WT Sense Target Labeling and Control Reagents kit (P/N 900652) was used for the preparation of labelled cDNA from 500ng of total RNA without rRNA reduction
|
| |
|
| Hybridization protocol |
Hybridisation of 5.5ug labelled cDNA was done overnight for 17 hours, at 60 rpm, at 45ºC in a Hybridisation Oven 640 (Affymetrix). The protocol was conducted as described in the Affymetrix Whole Transcript (WT) Sense Target Labeling Assay Manual
|
| Scan protocol |
Washing and staining of the arrays was done on a Affymetrix 450 fluidics stations using the protocol FS450_0001, as described in the Affymetrix Whole Transcript (WT) Sense Target Labeling Assay Manual. Arrays where then scanned on an Affymetrix 3000 7G scanner, as described in the Genechip Expression Analysis Technical Manual
|
| Description |
RMA - affy (unlogged scale): G121_C09_rat50.CEL (4_1)
|
| Data processing |
The data where normalized using RMA normalisation strategies using the MADMAX analysis pipeline pmid:21778530
|
| |
|
| Submission date |
Nov 07, 2014 |
| Last update date |
Jun 20, 2016 |
| Contact name |
vincent bloks |
| Organization name |
UMCG
|
| Department |
Pediatrics
|
| Lab |
Pediatrics
|
| Street address |
Hanzeplein1
|
| City |
Groningen |
| ZIP/Postal code |
9713GZ |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL11534 |
| Series (1) |
| GSE63096 |
Malnutrition-associated hepatic steatosis and ATP depletion is caused by peroxisomal and mitochondrial dysfunction and rescued by fenofibrate |
|
