|
| Status |
Public on Mar 04, 2016 |
| Title |
Activated T normal rep3 |
| Sample type |
RNA |
| |
|
| Source name |
in vitro activated T cells from 5-week-old p53 KO mouse
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57Bl/6
genotype/variation: p53 KO
age: 5 weeks
tissue/cell type: in vitro activated, normal T cells
|
| Treatment protocol |
Lymphoma cells or normal cells as control were purified by cell sorting from liver or spleen.
|
| Growth protocol |
p53 deficient mice injected every 2 weeks with PBS or Heat-killer Streptococcus pneumoniae until disease development
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were extract with with the Qiagen RNeasy minikit (Qiagen), then 50 ng of total RNA were amplified using GeneChip 3' IVT Express Kit from Affymetrix.
|
| Label |
biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
As per manufacturer (Affymetrix)
|
| Scan protocol |
The Arrays were scanned using the Gene Chip Scanner 3000. Then CEL files were generated using the Affymetrix GeneChip Command Console software (AGCC) 3.0
|
| Description |
SAMPLE 18
|
| Data processing |
CHP files were processed using MAS 5.0 analysis algorithm with Affymetrix Expression Console software
affymetrix-algorithm-name = ExpressionStat
affymetrix-algorithm-version = 5.0
program-name = Expression Console
program-id = 1.2.1.20
|
| |
|
| Submission date |
Nov 13, 2014 |
| Last update date |
Mar 05, 2016 |
| Contact name |
GENESTIER Laurent |
| E-mail(s) |
laurent.genestier@inserm.fr
|
| Organization name |
INSERM
|
| Street address |
Faculté de Medecine Lyon Sud. 165 Chemin du petit Revoyet
|
| City |
Oullins |
| ZIP/Postal code |
69921 |
| Country |
France |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE63264 |
Expression of peripheral T lymphoma cells from p53 deficient |
| GSE174257 |
Expression data from resting and activated murine T cell as well as murine peripheral T cell lymphomas |
|
