|
| Status |
Public on Dec 18, 2014 |
| Title |
AIL-G4-499 |
| Sample type |
RNA |
| |
|
| Source name |
SKIN
|
| Organism |
Mus musculus |
| Characteristics |
strain: AIL
disease status: EBA
eba severity score: 4.4
onset week: 4
|
| Treatment protocol |
Experimental EBA was induced by immunization with an immune-dominant peptide within the murine NC1 domain of Collagen type VII (GST-mCOL7C) as previously described (Ludwig et al. 2011). In total, 600 mice of the AIL were immunized out of which 100 mice were randomly selected for miRNA expression profiling. Mice were evaluated every 4th week after immunization regarding their development and extent of skin disease, following an established scoring system for a total period of 12 weeks (Sitaru et al. 2006). Murine skin tissues were obtained for analysis at the end of the experiment.
|
| Growth protocol |
The outbred four-way autoimmune-prone advanced intercross line (AIL) was generated (in our group) from the parental mouse strains BXD2/TyJ, MRL/MpJ, NZM2410/J and CAST/EiJ (Asghari et al. 2011; Ludwig et al. 2012; Srinivas et al. 2013). All strains were purchased from The Jackson Laboratory (Bar Harbor, ME). The four inbred strains were intercrossed following an equal strain and sex distribution. First generation (G1) offspring mice were then mated based on their parental origin to generate G2 mice in order to maintain an equal distribution of the original strain alleles across the genome. The same procedure was applied for intercrossing G2 and G3 mice. At least 50 breeding pairs were used for each successive generation of mice. Animals were held under pathogen free conditions at a 12-hour light/dark cycle with food and water ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from skin tissue and processed as previously described using the Flash Tag Biotin HSR RNA labeling kit
|
| Label |
biotin
|
| Label protocol |
according to the manufacturing guidelines (Affymetrix)
|
| |
|
| Hybridization protocol |
according to the manufacturing guidelines (Affymetrix)
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
|
| Description |
total RNA from 6 month old mice in G4 advance inter cross line
|
| Data processing |
R bioconductor 2.0
|
| |
|
| Submission date |
Dec 17, 2014 |
| Last update date |
Dec 18, 2014 |
| Contact name |
Saleh M. Ibrahim |
| E-mail(s) |
Saleh.Ibrahim@uksh.de
|
| Phone |
+49 451 500 5250
|
| Organization name |
Universität zu Lübeck
|
| Department |
Dermatology
|
| Street address |
Ratzeburger Allee 160
|
| City |
Lübeck |
| State/province |
Schleswig-Holstein |
| ZIP/Postal code |
23562 |
| Country |
Germany |
| |
|
| Platform ID |
GPL8786 |
| Series (1) |
| GSE64276 |
Dissecting the genetics of cutaneous miRNA in a mouse model of autoimmune bullous disease |
|
