|
| Status |
Public on Jul 06, 2021 |
| Title |
Kidney_RapKD_1week_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Kidney_RapKD_1week
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6 N
genotype/variation: Raptor fl/fl*KspCre
gender: female
age: 1 week
tissue: kidney
|
| Treatment protocol |
Kidneys were split in half and immediately snap frozen in liquid nitrogen.
|
| Growth protocol |
Raptor fl/fl or Kif fl/fl mice (used as controls), Raptor fl/fl*KspCre, Kif3a fl/fl*KspCre and Raptor fl/fl*Kif3a fl/fl*KspCre animals were sacrificed at 1 day, 1 week, 2 and 4 weeks (n>=7).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Plus Kit (Qiagen, Hilden, Germany)
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA was generated from 1.5 µg total RNA using the One-Cycle Target Labeling Assay (Affymetrix).
|
| |
|
| Hybridization protocol |
15 µg labelled cRNA were hybridized onto Mouse 4302 arrays (Affymetrix, CA, USA) for 16h at 45°C using Hybridization Wash and Stain Kit (Affymetrix)
|
| Scan protocol |
Arrays were washed and stained with GeneChip Fluidics Station 450 and scanned with GeneChip Scanner 3000 using the GeneChip Operating Software (Affymetrix).
|
| Description |
Kidney_RapKD_1week_01
|
| Data processing |
R/Bioconductor using Single-Channel Array Normalization (SCAN), (Picolo et al., Genomics 2012).
|
| |
|
| Submission date |
Dec 31, 2014 |
| Last update date |
Jul 06, 2021 |
| Contact name |
Hauke Busch |
| E-mail(s) |
hauke.busch@uni-luebeck.de
|
| Phone |
+49-451-3101-8470
|
| Organization name |
University of Lübeck
|
| Department |
Lübeck Institute of Experimental Dermatology
|
| Street address |
Ratzeburger Allee 160
|
| City |
Lübeck |
| State/province |
Schleswig-Holstein |
| ZIP/Postal code |
23538 |
| Country |
Germany |
| |
|
| Platform ID |
GPL19559 |
| Series (1) |
| GSE58044 |
mTORC1-mediated growth of kidney cysts is rapidly bypassed by other signaling pathways |
|
