|
| Status |
Public on Jul 01, 2015 |
| Title |
MWF_E15.5_kidney4 [exon-level] |
| Sample type |
RNA |
| |
|
| Source name |
MWF-E15.5 kidney
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: MWF
age: E15.5
tissue: kidney
|
| Treatment protocol |
not applicable
|
| Growth protocol |
Rat embryonic kidney dissected at day E15.5/E16.0 respectively
|
| Extracted molecule |
total RNA |
| Extraction protocol |
embryonic kidneys were harvested, snap-frozen in liquid nitrogen and stored at -80°C. Total RNA preparations of male kidney tissues were performed for MWF rats at developmental stage E15.5 and SHRs at E16.0 (n=4) using the RNeasy Micro Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. The quality and concentration of the purified total RNA were confirmed by spectroscopy.
|
| Label |
biotin
|
| Label protocol |
Terminal labeling kits (Ambion, New-York, USA)
|
| |
|
| Hybridization protocol |
Arrays were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
Affymetrix GeneChip® Scanner 3000 7G using GeneChip® Operating Software (GCOS) for image processing
|
| Description |
LEO-MWF-04-Leo_363-220212-RatGe-St1-0.CEL
|
| Data processing |
After normalization the arrays have been checked for outliers by principal component analysis (PCA), using a correlation dispersion matrix and normalized Eigenvector scaling. The analysis of differential expression of summarized gene level expression has been performed with Partek Genomic Suite version 6.5
RaGene-1_0-st-v1.r4.pgf
RaGene-1_0-st-v1.r4.mps
|
| |
|
| Submission date |
Feb 19, 2015 |
| Last update date |
Jul 01, 2015 |
| Contact name |
Reinhold Kreutz |
| Organization name |
Charité
|
| Street address |
Charitéplatz 1
|
| City |
Berlin |
| ZIP/Postal code |
10117 |
| Country |
Germany |
| |
|
| Platform ID |
GPL10741 |
| Series (1) |
| GSE66079 |
Expression data of embryonic kidney from MWF E15.5 and SHR 16.0 rats |
|
| Relations |
| Alternative to |
GSM1614018 (gene-level analysis) |
