|
| Status |
Public on May 15, 2015 |
| Title |
Group 1: Mouse control 5, biological rep 5 |
| Sample type |
RNA |
| |
|
| Source name |
Control mouse, without injury
|
| Organism |
Mus musculus |
| Characteristics |
strain: B6D2F1/J
tissue: Serum
age: 10-12 weeks
weigh: 22-26g
Sex: female
treatment: none
|
| Treatment protocol |
All radiation and burn injuries will be performed at AFRRI Radiation and Animal facility as per IACUC approved protocols. 30 days following combined radiation and burn injuries arterial blood will be harvested from euthanized mice for genomic microRNA analysis.
|
| Growth protocol |
B6D2F1/J female mice will be obtained from The Jackson Laboratory, Bar Harbor, Maine.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Mouse 200ul serum samples were subjected to total RNA isolation procedure using SeraMir Exosome RNA Amplification kit (System Biosciences, Mountain View, CA) according to manufacturer’s instructions. Concentration of each RNA sample was determined by NanoDrop® spectrophotometer ND-1000 (NanoDrop Technologies, Wilmington, DE). The quality of RNA samples was assessed with Agilent 2100 Bioanalyzer (Agilent Technologies Inc., Santa Clara, CA).
|
| Label |
biotin
|
| Label protocol |
Exosomal RNA samples, containing low molecular weight RNA, were biotin-labeled with FlashTag™ Biotin HSR RNA Labeling Kit. The quality of the biotin labeling process was confirmed by using Enzyme Linked Oligosorbent Assay (ELOSA) protocol (Affymetrix, Santa Clara, CA).
|
| |
|
| Hybridization protocol |
21.5ul of each high-quality biotin-labeled miRNA sample was hybridized to Affymetrix Gene-Chip® miRNA 2.0 Array for 16 hours according to Affymetrix protocol. The arrays were washed and stained on the Affymetrix Fluidics station 400.
|
| Scan protocol |
The arrays were scanned with a Hewlett Packard G2500A gene Array Scanner.
|
| Description |
Serum exosomal microRNA gene expression data in control mouse
|
| Data processing |
Gene expression values were obtained using Affymetrix Expression Console software, checked on Quality Control, normalized and analyzed using RMA-DABG option (Affymetrix, Santa Clara, CA).
|
| |
|
| Submission date |
Mar 10, 2015 |
| Last update date |
May 15, 2015 |
| Contact name |
Svetlana Ghimbovschi |
| Organization name |
Children's National Medical Center
|
| Department |
Research Center for Genetic Medicine
|
| Lab |
Dr. Hoffman's Lab.
|
| Street address |
111 Michigan Ave., NW
|
| City |
Washington |
| State/province |
DC |
| ZIP/Postal code |
20010 |
| Country |
USA |
| |
|
| Platform ID |
GPL16384 |
| Series (1) |
| GSE66739 |
Identifying Non-Invasive Molecular Correlates relevant to Irradiation-Skin Burn Combined Injuries |
|
