|
| Status |
Public on May 07, 2015 |
| Title |
TSEC 767- biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Murine Liver Sinusoidal Endothelial Cell Line (TSEC)
|
| Organism |
Mus musculus |
| Characteristics |
treatment: 767
cell type: Murine Liver Sinusoidal Endothelial Cell Line
|
| Treatment protocol |
Cells were treated 0.66mM Oleic Acid and 0.33mM Palmitic Acid and/or 30uM INT-767 for 8 hours.
|
| Growth protocol |
TSECs were plated at 1,000,000 cells per well in a 24 well plate.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of RNA was performed using QIAgen RNeasy Kit according to manufacturers protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (GeneChip WT PLUS Reagent Kit User Manual, 2013, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 3.5mg of fragmented and labeled ss-DNA were hybridized for 16h at 45C with 60rpm rotation on GeneChip® Mouse Gene 2.0 ST Array per protocol (GeneChip WT PLUS Reagent Kit User Manual, 2013, Affymetrix). GeneChips were washed and stained in the Affymetrix Fluidics Station 450 per protocol (GeneChip WT PLUS Reagent Kit User Manual, 2013, Affymetrix).
|
| Scan protocol |
GeneChips were scanned using Affymetrix® GeneChip® Scanner 3000
|
| Data processing |
Partek Genomics Suite v6.6 was used to import and process these data using quantile normalization and RMA background correction adjusting for GC content. Only those probes with log2 expression greater than 4.5 in at least one sample (26,084 out of 41,353 total) were used for subsequent statistical analysis.
|
| |
|
| Submission date |
Apr 07, 2015 |
| Last update date |
May 07, 2015 |
| Contact name |
Michael Edwards |
| E-mail(s) |
michael.edwards@ucdenver.edu
|
| Phone |
303-724-6054
|
| Organization name |
UC Denver
|
| Department |
Pulmonary
|
| Street address |
12700 East 19th Avenue, Box C272
|
| City |
Aurora |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE67651 |
Expression data from fatty acid and bile acid receptor agonist treated liver sinusoidal endothelial cells |
|
