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Sample GSM16644 Query DataSets for GSM16644
Status Public on Feb 11, 2004
Title RatLung_Sh_6
Sample type RNA
 
Channel 1
Source name Lung of sham animal
Organism Rattus norvegicus
Extracted molecule total RNA
 
Channel 2
Source name Pooled RNA of equal amounts from 10 organs (liver, lung, kidney, spleen, heart, skeletal muscle, skin, jejunum, and brain) of untreated control animals
Organism Rattus norvegicus
Extracted molecule total RNA
 
 
Description Male Sprague-Dawley rats weighing 280-300 g were anesthetized with 1.75% to 2.5% isofluorane and instrumented with two arterial catheters, one externalized for blood withdrawal and the other (not externalized) attached to the Data Sciences International (DSI) PhysioTel (St. Paul, MN, model C50-PXT) monitoring device placed intraperitoneally and used in monitoring blood pressure, temperature, and electrocardiogram data via telemetry. After 7 to 10 days of recovery, and only after the animals had begun to gain weight, the experiment was performed. At the beginning of hemorrhage (H), blood was withdrawn manually through the externalized catheter with 1-ml syringes at l ml/min. Total blood volume was estimated to be 6.5% of the body weight and 40% of this (2.6 ml/100g) was removed to effect the hemorrhage.
Keywords = rat lung hemorrhage
 
Submission date Feb 06, 2004
Last update date May 28, 2005
Contact name Phillip Bowman
E-mail(s) phillip.bowman@cen.amedd.army.mil
Phone 210-916-4297
Organization name Institute of Surgical Research
Street address 3400 Rawley E. Chambers Ave
City San Antonio
State/province TX
ZIP/Postal code 78234-6315
Country USA
 
Platform ID GPL968
Series (1)
GSE1034 Temporal Response of Rat Lung to Hemorrhage

Data table header descriptions
ID_REF
VALUE log ratio (log2 of PRE_VALUE)
N635_MEDIAN The normalized median intensity of Cy5 calculated by subtracting the Cy5 median background intensity taken from a standard area around the spot from the Cy5 emission in the pixels at each spot after laser excitation, and normalized to their overall mean, and then re-scaled to produce an overall geometric mean Cy5/Cy3 ratio of 1 for the array.
N532_MEDIAN The normalized median intensity of Cy3 calculated by subtracting the Cy3 median background intensity taken from a standard area around the spot from the Cy3 emission in the pixels at each spot after laser excitation, and normalized to their overall mean, and then re-scaled to produce an overall geometric mean Cy5/Cy3 ratio of 1 for the array.
F635_MEDIAN Cy5 channel median intensity of the spot.
F635_MEAN Cy5 channel average intensity of the spot.
F635_SD The standard deviation calculated for the Cy5 channel mean intensity of the spot.
B635_MEDIAN Cy5 channel background median intensity around the spot.
B635_MEAN Cy5 channel background average intensity around the spot.
B635_SD The standard deviation calculated for the Cy5 channel background average intensity around the spot.
F635_QUALITY Cy5 channel percentage of pixels in the spot more than one standard deviation above background.
F532_MEDIAN Cy3 channel median intensity of the spot.
F532_MEAN Cy3 channel average intensity of the spot.
F532_SD The standard deviation calculated for the Cy3 channel mean intensity of the spot.
B532_MEDIAN Cy3 channel background median intensity around the spot
B532_MEAN Cy3 channel background average intensity around the spot.
B532_SD The standard deviation calculated for the Cy3 channel background average intensity around the spot.
F543_QUALITY Cy3 channel percentage of pixels in the spot more than one standard deviation above background.
FLAGS The indication of spot quality determined by the image analysis software. Unflaged spot was assigned a value of zero. A negative value was assigned to a spot with intensity near background, uneven spot or background intensity.
PRE_VALUE The ratio of normalized median intensity of Cy5 (N635_MEDIAN) to normalized median intensity of Cy3 (N532_MEDIAN). These values were further processed before statistical analysis. Please refer to the paper and its supplement for the detailed data analysis.

Data table
ID_REF VALUE N635_MEDIAN N532_MEDIAN F635_MEDIAN F635_MEAN F635_SD B635_MEDIAN B635_MEAN B635_SD F635_QUALITY F532_MEDIAN F532_MEAN F532_SD B532_MEDIAN B532_MEAN B532_SD F543_QUALITY FLAGS PRE_VALUE
1 0.6599253 2.034361855 1.287864079 6654 6917 1853 490 667 1201 100 4842 5050 2058 1000 1254 2416 95 0 1.58
2 0.7484613 2.267737456 1.351888556 7445 7441 1880 575 853 2485 97 5225 5217 1306 1191 1584 3644 64 0 1.68
3 1.2078933 0.734126361 0.317775936 2750 2727 1051 525 948 3810 6 2056 2154 785 1108 1529 3798 1 0 2.31
4 1.3504973 0.753931929 0.295652295 2840 2967 1108 555 1177 4644 5 2061 2235 1106 1178 2168 6590 1 0 2.55
5 0.4329593 3.116075922 2.306557191 10024 9537 3150 583 694 558 99 8117 7768 2649 1235 1498 2197 90 0 1.35
6 0.2868813 2.819322506 2.309238844 9085 9077 2796 543 929 3640 95 7984 8005 2474 1095 1537 3652 91 0 1.22
7 0.4956953 0.930861663 0.65801072 3364 3296 1404 543 625 479 91 3027 3061 1104 1064 1211 859 89 0 1.41
8 0.6135323 1.037481634 0.679463948 3685 3704 1435 542 657 771 96 3106 3205 1511 1078 1308 1757 61 0 1.53
9 -0.2515393 0.372674758 0.444148856 1705 1764 759 575 1184 5132 0 2441 2481 779 1115 1779 4836 0 0 0.84
10 -0.4344033 0.36343216 0.490072172 1635 1774 904 533 728 1381 39 2555 2662 908 1093 1371 2276 10 0 0.74
11 0.1375043 0.370364109 0.337553131 1611 1719 797 489 605 688 78 1988 2246 2845 980 1192 1521 13 0 1.10
12 0.2750073 0.444304893 0.368392145 1798 1859 770 451 557 552 87 2014 2075 531 914 1082 1469 23 0 1.21
13 -0.1046973 0.328772417 0.354983878 1452 1522 756 455 526 405 81 1961 1965 540 902 993 760 70 0 0.93
14 -0.1844253 0.395121068 0.446495303 1659 1740 882 462 1038 4797 0 2243 2302 647 911 1673 5534 0 0 0.88
15 0.4854273 0.344616871 0.245371293 1503 1643 1029 459 644 1303 39 1623 1777 1187 890 1136 1936 5 0 1.40
16 0.5058913 0.433741924 0.305373289 1702 1754 748 388 438 319 93 1706 1734 466 795 815 323 94 0 1.42
17 0.0285693 0.43440211 0.427053315 1839 1919 935 523 642 730 77 2349 2441 1342 1075 1238 1183 55 0 1.02
18 0.3785123 0.517585492 0.397890334 2130 2250 1326 562 705 954 74 2401 2566 1016 1213 1429 1863 18 0 1.30
19 0.6780723 1.086665459 0.679128741 3900 3812 1263 607 715 574 96 3343 3372 841 1317 1415 975 90 0 1.60
20 0.5753123 0.94109454 0.632199806 3399 3431 1009 548 844 2968 44 2973 2991 646 1087 1332 2601 13 0 1.49

Total number of rows: 13056

Table truncated, full table size 1353 Kbytes.




Supplementary data files not provided
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