|
| Status |
Public on May 17, 2016 |
| Title |
AI992 |
| Sample type |
RNA |
| |
|
| Source name |
Not Inseminated
|
| Organism |
Sus scrofa |
| Characteristics |
tissue: oviductal ampullary-isthmic junction
gender: female
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the ‘TRIzol method’ according to the protocol recommended by the manufacturer (Life Technologies Inc., Gaithersburg, MD). The quantity and quality of the RNA samples were analysed with ARN 6.000 NanoLabChip® kit and the Agilent 2100 Bionalyzer (Agilent Technologies, Santa Clara, CA) respectively. Only samples with an RNA integrity number of > 7.0 were used for microarray analysis.
|
| Label |
Cy3
|
| Label protocol |
One-color microarray-based GE LowInput QuickAmp Labeling (Agilent)
|
| |
|
| Hybridization protocol |
Microarrays where hybridized and washed according to manufacturer's protocol
|
| Scan protocol |
Scanned on GenePix 4100A 01 [109997]
|
| Description |
Gene expression whitout insemination
|
| Data processing |
Images were quantified using GenePix Pro 6.0.1.25.
|
| |
|
| Submission date |
Apr 22, 2015 |
| Last update date |
May 17, 2016 |
| Contact name |
Pilar Coy |
| E-mail(s) |
pcoy@um.es
|
| Phone |
34868884789
|
| Organization name |
University of Murcia
|
| Department |
Physiology
|
| Lab |
Physiology of Reproduction
|
| Street address |
Campus de Espinardo.Facultad de Veterinaria
|
| City |
Murcia |
| State/province |
Murcia |
| ZIP/Postal code |
30100 |
| Country |
Spain |
| |
|
| Platform ID |
GPL15007 |
| Series (1) |
| GSE68148 |
Oviductal transcriptome is modified after insemination during spontaneous ovulation in the sow |
|
