|
| Status |
Public on May 16, 2015 |
| Title |
retina/RPE_wildtype_1year_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
retina/RPE_wildtype_1year
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57Bl/6J
genotype/variation: wild type
gender: male
age: 1 year
tissue: Retina/Retinal Pigmented Epithelium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Wild-type and nm3342 mice at P30 and P365 (n = 4/timepoint) were sacrificed, after which mRNA was isolated from both retinas and RPE using a mirVana RNA isolation kit (Life Technologies, Carlsbad, CA).
|
| Label |
Cy3
|
| Label protocol |
RNA quality was assessed with a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). Samples with an RNA Integrity Number (RIN) greater or equal to 8.0 were deemed acceptable and used for microarray anaylsis. Samples were prepared following Agilent’s One-Color Microarray-Based Gene Expression Analysis Low Input Quick Amp Labeling Kit v6.0
|
| |
|
| Hybridization protocol |
Samples with an RNA Integrity Number (RIN) greater or equal to 8.0 were deemed acceptable and used for microarray anaylsis. Samples were prepared following Agilent’s One-Color Microarray-Based Gene Expression Analysis Low Input Quick Amp Labeling Kit v6.0. An input of 100ng of total RNA was used to generate Cyanine-3 labeled cRNA. Samples were subsequently hybridized on Agilent SurePrint G3 Mouse GE 8 X 60K Microarray (Design ID: 028005)
|
| Scan protocol |
Arrays were scanned using an Agilent DNA Microarray Scanner at a 3um scanning resolution
|
| Description |
Gene expression of age matched wildtype
|
| Data processing |
Data was processed with Agilent Feature Extraction 11.0.1.1. Processed signal was normalized using Agilent GeneSpring 12.0
|
| |
|
| Submission date |
May 15, 2015 |
| Last update date |
May 16, 2015 |
| Contact name |
Steven K Fisher |
| E-mail(s) |
fisher@lifesci.ucsb.edu
|
| Phone |
805-893-3611
|
| Organization name |
University of California, Santa Barbara
|
| Department |
Neuroscience Research Institute
|
| Lab |
Retinal Cell Biology
|
| Street address |
BLDG. 571 Lagoon Rd.
|
| City |
Santa Barbara |
| State/province |
CA |
| ZIP/Postal code |
93106 |
| Country |
USA |
| |
|
| Platform ID |
GPL10787 |
| Series (1) |
| GSE68961 |
Characterization of Anatomical, Macromolecular, and Gene Expression Changes in the Retinal and Retinal Pigmented Epithelium of the nm3342 Mouse: A Potential Model of Serous Retinal Detachment |
|
