|
| Status |
Public on Jun 30, 2016 |
| Title |
Patient 8 |
| Sample type |
RNA |
| |
|
| Source name |
Patient 8
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: blood serum
Stage: patients with FIGO stages III/IV
condition: Ovarian cancer patient
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Small RNAs were isolated using the Macherey-Nagel NucleoSpin® miRNA Plasma kit
|
| Label |
biotin
|
| Label protocol |
Small RNAs were labelled with Flash Tag Biotin HSR kit according to manufacter's (Genisphere) recommendations.
|
| |
|
| Hybridization protocol |
Then samples were hybridized during 16 hours at 48°C on GeneChip microRNA V4 arrays of Affymetrix.
|
| Scan protocol |
Scan performed with scanner GC30007G (Affymetrix).
|
| Data processing |
Raw data were normalized using Expression Console (Affymetrix) based on RMA method.
|
| |
|
| Submission date |
May 28, 2015 |
| Last update date |
Jun 30, 2016 |
| Contact name |
Christophe DENOYELLE |
| E-mail(s) |
c.denoyelle@baclesse.fr
|
| Phone |
332 31 45 51 71
|
| Organization name |
Université de Caen Normandie
|
| Department |
Centre de Lutte Contre le Cancer François Baclesse
|
| Lab |
UMR Inserm 1199 - Unité BioTICLA (Biologie et Thérapies Innovantes des Cancers Localement Agressifs)
|
| Street address |
3 Avenue général Harris BP 5026
|
| City |
Caen cedex 05 |
| ZIP/Postal code |
14076 |
| Country |
France |
| |
|
| Platform ID |
GPL19117 |
| Series (1) |
| GSE69341 |
Towards a new standardized method for circulating miRNAs profiling in clinical studies: Interest of the exogenous normalization to improve miRNA signature accuracy |
|
