|
| Status |
Public on Mar 25, 2016 |
| Title |
HepG2_6h_DMSO.S32B1G181 |
| Sample type |
RNA |
| |
|
| Source name |
HepG2 Human Hepatocyte Carcinoma Cell Line
|
| Organism |
Homo sapiens |
| Characteristics |
chemical treatment: Clobetasol
dose: 100uM
replicate: 2
gender: Male
cell line: HepG2
|
| Treatment protocol |
Cells (5X10E5 cells/well in 12 well cell culture plates (Corning, Corning ,NY) ) were treated with 3 concentrations of chemical or DMSO vehicle for 6 h.
|
| Growth protocol |
HepG2 human hepatocellular carcinoma cells were purchased from American Type Culture Collection (Manassas, VA) and grown in Eagle’s Minimum Essential Meidum (EMEM) containing phenol red (ATCC, Manassas, VA) supplemented with 10% fetal bovine serum (FBS) (ATCC), 100 U/ml penicillin and 100 μg/ml streptomycin (ATCC) and maintained at 37°C in an atmosphere of 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were extracted in QIAgen RLT buffer and total RNA was extracted using the Agencourt RNAdvance Tissue protocol implemented on a Beckman FXp robot using methods provided by Beckman Coulter.
|
| Label |
biotin
|
| Label protocol |
500 ng of total RNA was converted to Affymetrix GeneChip targets using the Affymetrix IVT-Express kit and protocols provided by Affymetrix.
|
| |
|
| Hybridization protocol |
GeneChip targets were hybridized on Affymetrix U219-96 plates using the GeneTitan single-channel instrument and protocols provided by Affymetrix.
|
| Scan protocol |
GeneTitan plates were scanned on a GeneTitan single-channel instrument using protocols provided by Affymetrix.
|
| Description |
Chemical signature/does response study
|
| Data processing |
Data was processed using the PLIER algorithm implemented from the Affymetrix Command Console Software.
|
| |
|
| Submission date |
Jun 12, 2015 |
| Last update date |
Mar 26, 2016 |
| Contact name |
Jay Patrick Tiesman |
| Organization name |
Procter & Gamble
|
| Department |
Global Biotechnology
|
| Lab |
Mason Business Center
|
| Street address |
PO BOX 8006
|
| City |
MASON |
| State/province |
OH |
| ZIP/Postal code |
45040-8006 |
| Country |
USA |
| |
|
| Platform ID |
GPL13667 |
| Series (2) |
| GSE69850 |
Expression data for HepG2 cells treated with 34 different chemicals |
| GSE69851 |
Human toxicology-relevant cell lines treated with three doses of 34 chemical compounds |
|
