|
| Status |
Public on Jul 01, 2016 |
| Title |
4 Week-old WT testis_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
4 week-old WT
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: testis
age: 4 week old
genotype: WT
|
| Treatment protocol |
Nothing was treated.
|
| Growth protocol |
Pups were stayed with their biological mother.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNA easy mini kit.
|
| Label |
Cy3
|
| Label protocol |
Starting with 500ng of total RNA, Cy3 labeled cRNA was produced according to manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
For each sample, 1.65ug of Cy3 labeled cRNAs were fragmented and hybridized for 17 hours in a rotating hybridization oven.
|
| Scan protocol |
Slides were washed and then scanned with an Agilent Scanner.
|
| Description |
RNA samples from 4 week-old WT
|
| Data processing |
Data was obtained using the Agilent Feature Extraction software (v9.5), using the 1-color defaults for all parameters.
|
| |
|
| Submission date |
Jun 23, 2015 |
| Last update date |
Jul 01, 2016 |
| Contact name |
NIEHS Microarray Core |
| E-mail(s) |
microarray@niehs.nih.gov, liuliw@niehs.nih.gov
|
| Organization name |
NIEHS
|
| Department |
DIR
|
| Lab |
Microarray Core
|
| Street address |
111 T.W. Alexander Drive
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL4134 |
| Series (2) |
| GSE70194 |
Glis3 plays a crucial role in spermatogenesis [848_827] |
| GSE70196 |
Glis3 plays a crucial role in spermatogenesis |
|
