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Status |
Public on Jan 13, 2017 |
Title |
377 |
Sample type |
RNA |
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Source name |
subcutaneous adipose tissue
|
Organism |
Homo sapiens |
Characteristics |
tissue: subcutaneous adipose tissue gender: male age: 60 bmi: 25.7275552140065 whr: 0.908629441624365 waist_circumference: 89.5 hip_circumference: 98.5 ffmass: 80.7 totfa: 22.70756 p_adipon: 8 matsuda: 3.84208427871299 p_crp: 0.625 p_il1ra: 106.15 gfr: 0.0994619152995335 p_ffa0: 0.85 s_tottg: 2.82 s_totalc: 6.06 s_ldlc: 3.73 s_hdlc: 1.72 homair: 3.04177777777778 p_gl0: 5.9 p_ins0: 11.6 p_proi0: 11 b_ghba1c: 5.58211095036 systbp: 150 diastbp: 82.6666666666667
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiazol with homogenization followed by Rneasy spin columns with option Dnase per manufacturers protocol
|
Label |
biotin
|
Label protocol |
All target labeling reagents were purchased from Affymetrix (Santa Clara, CA). Double-stranded cDNAs were synthesized from 1ug total RNA through reverse transcription with an oligo-dT primer containing the T7 RNA polymerase promoter and double strand conversion using the cDNA Synthesis System. Biotin-labeled cRNA was generated from the cDNA and used to probe Affymetrix Mouse Genome HT_MG-430A arrays.
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Hybridization protocol |
Array hybridization, washing and scanning were performed according to the manufacturer’s recommendations.
|
Scan protocol |
Scanned images were subjected to visual inspection and a chip quality report was generated by the Affymetrix's GeneChip Operating System (GCOS) and Expression console (Affymetrix).
|
Data processing |
The image data was processed using the Affymetrix GCOS algorithm utilizing quantile normalization or the Robust Multiarray method (RMA) to determine the specific hybridizing signal for each gene.
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Submission date |
Jun 28, 2015 |
Last update date |
Jan 13, 2017 |
Contact name |
Calvin Pan |
Organization name |
UCLA
|
Street address |
675 Charles Young Dr South
|
City |
Los Angeles |
ZIP/Postal code |
90095 |
Country |
USA |
|
|
Platform ID |
GPL13667 |
Series (1) |
GSE70353 |
Subcutaneous adipose tissue gene expression from men that are part of the METSIM study |
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