|
| Status |
Public on Jan 13, 2017 |
| Title |
987 |
| Sample type |
RNA |
| |
|
| Source name |
subcutaneous adipose tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: subcutaneous adipose tissue
gender: male
age: 55
bmi: 26.4828383182595
whr: 1.04102564102564
waist_circumference: 101.5
hip_circumference: 97.5
ffmass: 79.6
totfa: 11.51958
p_adipon: 24.1
matsuda: 14.6511804377976
p_crp: 4.315
p_il1ra: 134.75
gfr: 0.0851157808509451
p_ffa0: 0.34
s_tottg: 0.99
s_totalc: 6.68
s_ldlc: 4.36
s_hdlc: 1.87
homair: 0.521333333333333
p_gl0: 5.1
p_ins0: 2.3
p_proi0: 8
b_ghba1c: 5.9
systbp: 146.666666666667
diastbp: 104.666666666667
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiazol with homogenization followed by Rneasy spin columns with option Dnase per manufacturers protocol
|
| Label |
biotin
|
| Label protocol |
All target labeling reagents were purchased from Affymetrix (Santa Clara, CA). Double-stranded cDNAs were synthesized from 1ug total RNA through reverse transcription with an oligo-dT primer containing the T7 RNA polymerase promoter and double strand conversion using the cDNA Synthesis System. Biotin-labeled cRNA was generated from the cDNA and used to probe Affymetrix Mouse Genome HT_MG-430A arrays.
|
| |
|
| Hybridization protocol |
Array hybridization, washing and scanning were performed according to the manufacturer’s recommendations.
|
| Scan protocol |
Scanned images were subjected to visual inspection and a chip quality report was generated by the Affymetrix's GeneChip Operating System (GCOS) and Expression console (Affymetrix).
|
| Data processing |
The image data was processed using the Affymetrix GCOS algorithm utilizing quantile normalization or the Robust Multiarray method (RMA) to determine the specific hybridizing signal for each gene.
|
| |
|
| Submission date |
Jun 28, 2015 |
| Last update date |
Jan 13, 2017 |
| Contact name |
Calvin Pan |
| Organization name |
UCLA
|
| Street address |
675 Charles Young Dr South
|
| City |
Los Angeles |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL13667 |
| Series (1) |
| GSE70353 |
Subcutaneous adipose tissue gene expression from men that are part of the METSIM study |
|
