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Status |
Public on Jan 13, 2017 |
Title |
1149 |
Sample type |
RNA |
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Source name |
subcutaneous adipose tissue
|
Organism |
Homo sapiens |
Characteristics |
tissue: subcutaneous adipose tissue gender: male age: 51 bmi: 26.2580728395905 whr: 0.907766990291262 waist_circumference: 93.5 hip_circumference: 103 ffmass: 78.6 totfa: 17.26073 p_adipon: 3.1 matsuda: 3.94237911798488 p_crp: 1.6 p_il1ra: 310.84 gfr: 0.0876049690229657 p_ffa0: 0.36 s_tottg: 3.45 s_totalc: 5.29 s_ldlc: 3.04 s_hdlc: 0.87 homair: 2.86 p_gl0: 6.5 p_ins0: 9.9 p_proi0: 16.1 b_ghba1c: 6.3 systbp: 132 diastbp: 85.3333333333333
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiazol with homogenization followed by Rneasy spin columns with option Dnase per manufacturers protocol
|
Label |
biotin
|
Label protocol |
All target labeling reagents were purchased from Affymetrix (Santa Clara, CA). Double-stranded cDNAs were synthesized from 1ug total RNA through reverse transcription with an oligo-dT primer containing the T7 RNA polymerase promoter and double strand conversion using the cDNA Synthesis System. Biotin-labeled cRNA was generated from the cDNA and used to probe Affymetrix Mouse Genome HT_MG-430A arrays.
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Hybridization protocol |
Array hybridization, washing and scanning were performed according to the manufacturer’s recommendations.
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Scan protocol |
Scanned images were subjected to visual inspection and a chip quality report was generated by the Affymetrix's GeneChip Operating System (GCOS) and Expression console (Affymetrix).
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Data processing |
The image data was processed using the Affymetrix GCOS algorithm utilizing quantile normalization or the Robust Multiarray method (RMA) to determine the specific hybridizing signal for each gene.
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Submission date |
Jun 28, 2015 |
Last update date |
Jan 13, 2017 |
Contact name |
Calvin Pan |
Organization name |
UCLA
|
Street address |
675 Charles Young Dr South
|
City |
Los Angeles |
ZIP/Postal code |
90095 |
Country |
USA |
|
|
Platform ID |
GPL13667 |
Series (1) |
GSE70353 |
Subcutaneous adipose tissue gene expression from men that are part of the METSIM study |
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