|
| Status |
Public on Dec 21, 2015 |
| Title |
SG-4 |
| Sample type |
RNA |
| |
|
| Source name |
Salivary gland
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: salivary gland
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After fresh tissues were homogenized by TissueLyser, total RNA was extracted by ISOGEN.
|
| Label |
digoxigenin
|
| Label protocol |
Digoxigenin-UTP-labelled cRNA was generated and amplified from 1 mg of total RNA using Applied Biosystems Chemiluminescent RT-IVT Labelling Kit.
|
| |
|
| Hybridization protocol |
Ten mg of labelled cRNA was hybridized to each pre-hybridized microarray at 55℃ for 16 hours.
|
| Scan protocol |
After Chemiluminescence detection using Applied Biosystems Chemiluminescence Detection Kit, images were collected by the AB1700 Chemiluminescent Microarray Analyzer.
|
| Data processing |
The quantile method was used for background correction and normalization.
|
| |
|
| Submission date |
Jul 07, 2015 |
| Last update date |
Dec 21, 2015 |
| Contact name |
Koh-ichi Nakashiro |
| E-mail(s) |
nakako@m.ehime-u.ac.jp
|
| Phone |
+81899605393
|
| Organization name |
Ehime University Graduate School of Medicine
|
| Department |
Oral and Maxillofacial Surgery
|
| Street address |
454 Shitsukawa
|
| City |
Toon |
| State/province |
Ehime |
| ZIP/Postal code |
791-0295 |
| Country |
Japan |
| |
|
| Platform ID |
GPL2986 |
| Series (1) |
| GSE70604 |
Gene expression profiles in lymph nodes with metastasis of oral squamous cell carcinoma (OSCC) |
|
