|
| Status |
Public on Jul 23, 2015 |
| Title |
WB_non-statin_pt_30 |
| Sample type |
RNA |
| |
|
| Source name |
PAXgene whole blood mRNA
|
| Organism |
Homo sapiens |
| Characteristics |
statin user (y/n): N
disease: COPD
age: 71
Sex: F
smoking status: Former smoker
fev1% predicted: 54.7
fev1/fvc: 49.98
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen PAXgene Blood miRNA Kit (Cat# 763134), automated processing using the Qiagen QIAcube robotic workstation (Cat# 9001292). Protocol to amplify RNA-Ambion WT Expression RNA Amplification Kit (Part# 4440536)
|
| Label |
biotin
|
| Label protocol |
Affymetrix GeneChip HT WT Terminal Labeling Kit (Part# 901648)
|
| |
|
| Hybridization protocol |
Affymetrix GeneTitan Hybridization, Wash and Stain Kit for WT Array Plates (Part# 901622)
|
| Scan protocol |
Affymetrix GeneChip Command Console (AGCC) version 4.1.2.1567G for scanning, image acquisition and image processing on the Affymetrix GeneTitan Multi-Channel(MC) Instrument.
|
| Data processing |
RMA normalization with Farms bioconductor package to filter out non-informative probe sets
|
| |
|
| Submission date |
Jul 22, 2015 |
| Last update date |
Aug 16, 2016 |
| Contact name |
Bruce Maxwell McManus |
| E-mail(s) |
Bruce.McManus@hli.ubc.ca
|
| Phone |
604-806-8586
|
| Organization name |
PROOF Centre of Excellence
|
| Department |
UBC James Hogg Research Centre
|
| Street address |
1081 Burrard Street
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V6Z 1Y6 |
| Country |
Canada |
| |
|
| Platform ID |
GPL11532 |
| Series (1) |
| GSE71220 |
The effect of statins on blood gene expression in COPD |
|
